MHC Class I H2 Kd antibody [1.B.548] (ab62226)

Product Name 

MHC Class I H2 Kd antibody [1.B.548]

Product type 

Primary antibodies

Description 

Mouse monoclonal [1.B.548] to MHC Class I H2 Kd

Immunogen 

Full length MHC Class I H2 Kd protein (Mouse)

Reacts with 
(species key)

Ms

Specificity 

ab62226 detects classical mouse MHC class I antigen, H2 Kd allele.

Tested applications 
(see key)

Flow Cyt, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use 1µg for 10⁶ cells.
WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane

Research areas 

Immunology >> Adaptive Immunity >> MHC >> Class I

Relevance 

MHC Class I H2 Kd is involved in the presentation of foreign antigens to the immune system. The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.

MHC Class I H2 Kd is present on 100% of T-cells and B-cells including beta cells of Langerhans, etc. It is also present on endothelial and epithelial tissue.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

1.B.548

Isotype 

IgG2b

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 1.0% BSA, PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

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