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Products:Immunology >> Adaptive Immunity >> MHC >> Class I
Overview
Product name
Anti-MHC Class I H2 Kd antibody [34-1-2S]
See all MHC Class I H2 Kd products (5) ...
Description
Mouse monoclonal [34-1-2S] to MHC Class I H2 Kd
Specificity
ab25334 binds to a common determinant in the a3 domains of H-2Kd and H-2Dd in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Kb.
Tested applications
Flow Cyt, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
C3H.SW splenocytes (Mouse)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 100mM Borate buffered saline, pH 8.2
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
34-1-2S
Isotype
IgG2a
Light chain type
kappa
Research areas
Immunology >> Adaptive Immunity >> MHC >> Class I
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
IHC-Fr
Application notes
Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
Complement-mediated cytotoxicity: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
MHC Class I H2 Kd is involved in the presentation of foreign antigens to the immune system. The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
Cellular localization
Cell Membrane
Alternative names
- class I major histocompatibility antigen H 2Kd antibody
- H 2 class I histocompatibility antigen K D alpha chain [Precursor] antibody
- H 2K antibody
see all
Database links
- Entrez Gene: 14972 Mouse
- Entrez Gene: 14974 Mouse
- SwissProt: P01901 Mouse
- SwissProt: P01902 Mouse
- SwissProt: P03991 Mouse
- SwissProt: P04223 Mouse
References for Anti-MHC Class I H2 Kd antibody [34-1-2S] (ab25334)
This product has been referenced in:
- Allen Het al. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A 83:7447-51 (1986).Read more (PubMed: 3532114) »
- Allen Het al. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature 309:279-81 (1984).Read more (PubMed: 6201750) »
See all 4 publications for this product
Publishing research using ab25334? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"