MICA Human ELISA Kit (ab100592)
- Product nameMICA Human ELISA KitSee all MICA kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 20 pg/ml
- Range13.72 pg/ml - 10000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 116.9 109% - 124% Serum 112.3 95% - 129% Plasma 93.83 75% - 107%
- Species reactivityReacts with: Human
- Product overview
ab100592 Human MICA (MHC class I Chain-related gene A) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MICA in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human MICA coated on a 96-well plate. Standards and samples are pipetted into the wells and MICA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 300X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent 5x concentrated buffer. For Standard/Sample (serum/plasma samples/cell culture medium/urine) diluent. 1 x 15ml Detection Antibody MICA: biotinylated anti-human MICA 2 vials Human MICA Standard(recombinant) 2 vials MICA Microplate (12 strips x 8 wells) coated with anti-human MICA 1 unit Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- FLJ60820HLA class I antigen MGC111087
- MHC class I chain related gene A proteinMHC class I chain related protein AMHC class I polypeptide related sequence AMHC class I related proteinMIC A PERB11.1Stress inducible class I homolog
Our Abpromise guarantee covers the use of ab100592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for MICA Human ELISA Kit (ab100592)
ab100592 has not yet been referenced specifically in any publications.