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Overview

  • Product nameMICA Human ELISA KitSee all MICA kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 20 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 116.9 109% - 124%
    Serum 112.3 95% - 129%
    Plasma 93.83 75% - 107%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100592 Human MICA (MHC class I Chain-related gene A) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MICA in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human MICA coated on a 96-well plate. Standards and samples are pipetted into the wells and MICA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    300X HRP-Streptavidin Concentrate Item G 1 x 200µl
    Assay Diluent 5x concentrated buffer. For Standard/Sample (serum/plasma samples/cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody MICA: biotinylated anti-human MICA 2 vials
    Human MICA Standard(recombinant) 2 vials
    MICA Microplate (12 strips x 8 wells) coated with anti-human MICA 1 unit
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • RelevanceThe MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
  • Cellular localizationPlasma membrane
    • Alternative names
        FLJ60820HLA class I antigen MGC111087
        MHC class I chain related gene A proteinMHC class I chain related protein AMHC class I polypeptide related sequence AMHC class I related proteinMIC A PERB11.1Stress inducible class I homolog
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100592 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      MICA Human ELISA Kit images

      • ELISA - MICA Human ELISA Kit (ab100592)
        ELISA - MICA Human ELISA Kit (ab100592)

      Protocols

      References for MICA Human ELISA Kit (ab100592)

      ab100592 has not yet been referenced specifically in any publications.

      Publishing research using ab100592 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab100592 Submit a Question for ab100592

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      1) Extracellular domain of MICA not detected. Is the standard the recombinant full length MICA protein or a fragment?
      2) Standard values are much lower than the ones obtained with a kit from a competitor. The values are ok when the standard from the s...

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      Thank you for contacting us.

      Please find below the answers from our collaborator to your questions:

      1) Extracellular domain of MICA not detected. Is the standard the recombinant full length MICA protein or a fragment?
      The antibody pa...

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       Dear tech,   A customer would like to purchase a ELISA kit for soluble MICA. Do ab100592 and ab59569 match the need? Thank you for your help!   Best regards,  

        Thank you for your patient. Both products are suitable to use with soluble MICA.  However, please notice there are slightly differences between the kits. Ab100592 has been tested in human fluids, as Urine, Serum, and Plasma and also in Cell culture supe...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"