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Overview

  • Product nameMICA Human ELISA KitSee all MICA kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 6 2214.6pg/ml 50.3 2.3%
    B 6 1102.1pg/ml 39.5 3.6%
    C 6 641.6pg/ml 47.7 7.4%
    Inter-assay
    Sample n Mean SD CV%
    A 18 2416pg/ml 187.1 7.7%
    B 18 1200pg/ml 105.2 8.8%
    C 18 627pg/ml 50.9 8.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    = 123 pg/ml
  • Range
    156 pg/ml - 5000 pg/ml
  • Assay time
    3h 50m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MHC class I chain-related gene A glycoprotein (MICA) Human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of supernatant, buffered solutions or serum and plasma samples.

     

    Note: For serum and plasma quandification: high detection level is found in human serum and plasma samples. The high signal appears could be the result of the matrix and/or interaction with other molecules. Consequently serum and plasma quantification is under the scientist responsability.

     

    Principle

    The MICA Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for MICA has been coated onto the wells of the microtiter strips provided. Samples, including standards of known MICA concentrations and unknown are pipetted into these wells.

    During the first incubation, the MICA antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for MICA is

    incubated. Then the enzyme (streptavidin-horse radish peroxydase) is added. After incubation and washing to remove all unbound enzyme, a

    substrate solution of the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly

    proportional to the concentration of MICA present in the samples.

     

    Range: 156 pg/ml - 5000 pg/ml Sensitivity:

  • Notes

    Safety

    • For research use only.

     

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash

     

    thoroughly with water.

     

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

     

     

    • Do not pipette by mouth.

     

     

    • The stop solution in this kit contains 9.8% sulphuric acid.

     

  • Tested applicationsSandwich ELISA more details

Properties

  • RelevanceThe MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
  • Cellular localizationPlasma membrane
    • Alternative names
        FLJ60820HLA class I antigen MGC111087
        MHC class I chain related gene A proteinMHC class I chain related protein AMHC class I polypeptide related sequence AMHC class I related proteinMIC A MICAPERB11.1Stress inducible class I homolog
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab59569 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      MICA Human ELISA Kit images

      • ELISA - MICA ELISA Kit - 1x96 well Plate (ab59569)
        ELISA - MICA ELISA Kit - 1x96 well Plate (ab59569)

      References for MICA Human ELISA Kit (ab59569)

      ab59569 has not yet been referenced specifically in any publications.

      Publishing research using ab59569 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab59569 Submit a Question for ab59569

      Displaying 1 - 2 of 2 results for Abreviews and Q&A

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       Dear tech,   A customer would like to purchase a ELISA kit for soluble MICA. Do ab100592 and ab59569 match the need? Thank you for your help!   Best regards,  

        Thank you for your patient. Both products are suitable to use with soluble MICA.  However, please notice there are slightly differences between the kits. Ab100592 has been tested in human fluids, as Urine, Serum, and Plasma and also in Cell culture supe...

      Read More

      Are the following items included it the kit? 1) MICA clone AMO1 2) BAMO3 3) MCCA-04

      The clones mentionned below are not included in the Human MICA ELISA Kit.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"