Overview
Product nameMIF proteinSee all MIF proteins and peptides ...
Protein descriptionRecombinant full length Chicken MIF (amino acids 1-115) expressed in Yeast; 115 amino acids, Predicted MWt 12.5 kDa (Swiss-Prot Q02960).
Expression hostSaccharomyces cerevisiae
Properties
Purity> 95
% by SDS-PAGE
Purification notesab88204 was purified using Ion-exchange chromatography.
FormLyophilised:Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 10% Trehalose, 1X PBS
Concentration information loading...
Sequence notesMPMFTIHTNV CKDAVPDSLL GELTQQLAKA TGKPAQYIAV HIVPDQMMSF GGSTDPCALC SLYSIGKIGG QQNKTYTKLL CDMIAKHLHV SADRVYINYF DINAANVGWN GSTFA
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab88204
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
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Alternative names
GIFGLIFGlycosylation inhibiting factor
Glycosylation-inhibiting factorL-dopachrome isomeraseL-dopachrome tautomeraseMacrophage migration inhibitory factorMacrophage migration inhibitory factorMIFMIFMIF proteinMIF_HUMANMMIFPhenylpyruvate tautomerasePhenylpyruvate tautomerase
see all
FunctionPro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Involvement in diseaseGenetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Sequence similaritiesBelongs to the MIF family.
Cellular localizationSecreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
References for MIF protein (ab88204)
ab88204
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
