The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 53 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
FunctionInvolved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
Tissue specificityExpressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
Sequence similaritiesBelongs to the MINA53/NO66 family. Contains 1 JmjC domain.