Overview
- Product nameAnti-MINA53 antibodySee all MINA53 primary antibodies ...
- DescriptionRabbit polyclonal to MINA53
- SpecificityAt least three isoforms of MINA53 are known to exist; ab126282 will only recognize isoforms 1 and 3.
- Tested applicationsICC/IF, WB, IHC-P more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Synthetic peptide, corresponding to 15 N terminal amino acids of Human MINA53 (NP_694822).
- Positive controlHuman heart tissue lysate; Mouse heart tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab126282 is affinity chromatography purified via peptide column.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab126282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 20 µg/ml. |
| WB | WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 53 kDa. |
| IHC-P | IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
Target
- FunctionInvolved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
- Tissue specificityExpressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
- Sequence similaritiesBelongs to the MINA53/NO66 family.
Contains 1 JmjC domain. - Cellular localizationNucleus. Nucleus > nucleolus.
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Database links
- Entrez Gene: 84864 Human
- Entrez Gene: 67014 Mouse
- Omim: 612049 Human
- SwissProt: Q8IUF8 Human
- SwissProt: Q8CD15 Mouse
- Unigene: 655935 Human
- Unigene: 607776 Human
- Unigene: 289150 Mouse
Target information above from: UniProt accession
Q8IUF8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 60S ribosomal protein L27a histidine hydroxylase antibodyBifunctional lysine specific demethylase and histidyl hydroxylase MINA antibodyFLJ14393 antibody
- Histone lysine demethylase MINA antibodyMDIG antibodyMINA antibodyMINA_HUMAN antibodyMineral dust-induced gene protein antibodyMyc induced nuclear antigen 53 kDa antibodyMYC induced nuclear antigen antibodyMYC-induced nuclear antigen antibodyNO52 antibodyNucleolar protein 52 antibodyRibosomal oxygenase MINA antibodyROX antibody
see all
Anti-MINA53 antibody images
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Western blot - Anti-MINA53 antibody (ab126282)Lane 1 : Anti-MINA53 antibody (ab126282) at 1 µg/ml
Lane 2 : Anti-MINA53 antibody (ab126282) at 2 µg/ml
Lane 1 : Human heart tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 53 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MINA53 antibody (ab126282)ab126282, at 5 ug/ml, staining MINA53 in Paraffin-embedded Mouse heart tissue by Immunohistochemistry.
References for Anti-MINA53 antibody (ab126282)
ab126282 has not yet been referenced specifically in any publications.
