Rat MIP-1 beta ELISA Kit (CCL4) (ab171336)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat MIP-1 beta ELISA Kit (CCL4)
See all CCL4/MIP-1 beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 31pg/ml 1.42 = 4.6% 2 16 144pg/ml 9.21 = 6.4% 3 16 453pg/ml 24.31 = 5.5% Inter-assay Sample n Mean SD CV% 1 24 31pg/ml 1.67 = 5.4% 2 24 148pg/ml 12.13 = 8.2% 3 24 419pg/ml 24.3 = 5.8% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s rat MIP-1 beta (CCL4) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat MIP-1 beta in cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
A MIP-1 beta specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MIP-1 beta is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat MIP-1 beta amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat MIP-1 beta Antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-rat MIP-1 beta antibody 1 x 100µl Lyophilized recombinant rat MIP-1 beta standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all -
Database links
- Entrez Gene: 116637 Rat
- SwissProt: P50230 Rat
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab171336 has been referenced in 1 publication.
- Bi D et al. Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis. Exp Ther Med 14:2925-2930 (2017). PubMed: 28912850