Mouse MIP3 beta ELISA Kit (CCL19) (ab100729)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MIP3 beta ELISA Kit (CCL19)
See all MIP-3 beta/CCL19 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 5 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 85.6 78% - 101% Serum 107.7 82% - 132% Plasma 119.5 83% - 140% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s MIP3 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-3- beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP3 beta coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 260X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Mouse MIP3 beta 2 vials MIP3 beta Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP3 beta Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. -
Tissue specificity
Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta chemokine exodus 3
- Beta-chemokine exodus-3
- C C chemokine ligand 19
see all -
Database links
- Entrez Gene: 100039053 Mouse
- Entrez Gene: 24047 Mouse
- SwissProt: O70460 Mouse
- Unigene: 426373 Mouse
- Unigene: 441169 Mouse
- Unigene: 459263 Mouse
- Unigene: 6660 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab100729 has been referenced in 2 publications.
- Iida Y et al. Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells. J Immunother Cancer 8:N/A (2020). PubMed: 32675195
- Jenkins RW et al. Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids. Cancer Discov 8:196-215 (2018). PubMed: 29101162