Anti-MLF1 Interacting Protein/PBIP1 (phospho T78) antibody (ab34911)

Overview

• Product nameAnti-MLF1 Interacting Protein/PBIP1 (phospho T78) antibodySee all MLF1 Interacting Protein/PBIP1 primary antibodies ...
• Description
  Rabbit polyclonal to MLF1 Interacting Protein/PBIP1 (phospho T78)
• Tested applicationsWB, ELISA, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Cow
• Immunogen

  Synthetic peptide: PLHSTAIYAD, corresponding to amino acids 73-83 of Human MLF1 Interacting Protein

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Other

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Other

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin

Applications

Target

• FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
• Tissue specificityExpressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
• Post-translational
  modificationsPhosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
• Cellular localizationCytoplasm. Nucleus. Chromosome > centromere > kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.

Target information above from: UniProt accession Q71F23 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 617866 Cow
  • Entrez Gene: 79682 Human
  • Entrez Gene: 79682 Human
  • Entrez Gene: 71876 Mouse
  • Entrez Gene: 306464 Rat
  • SwissProt: Q2KIW5 Cow
  • SwissProt: Q9H5G1 Human
  • SwissProt: Q32Q71 Human

  • SwissProt: Q71F23 Human
  • SwissProt: Q71F23 Human
  • SwissProt: Q6UNA2 Mouse
  • SwissProt: Q9D9U1 Mouse
  • SwissProt: Q8C4M7 Mouse
  • SwissProt: Q4V8G7 Rat
  • Unigene: 55425 Cow
  • Unigene: 575032 Human
  • Unigene: 217385 Mouse
  • Unigene: 22108 Mouse
  • Unigene: 128609 Rat

  see all

• Alternative names
  CENP U antibodyCENP-50 antibodyCENP-U antibody

  CENP50 antibodyCENPU antibodyCENPU_HUMAN antibodyCENPU50 antibodycentromere protein of 50 kDa antibodycentromere protein U antibodyFLJ23468 antibodyHHV8 LNAIP1 antibodyICEN24 antibodyInterphase centromere complex protein 24 antibodyKaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1 antibodyKLIP-1 antibodyKLIP1 antibodyKSHV latent nuclear antigen interacting protein 1 antibodyKSHV latent nuclear antigen-interacting protein 1 antibodyKSHV LNAIP1 antibodyLNAIP 1 antibodyLNAIP-1 antibodyMLF1-interacting protein antibodyMLF1IP antibodyMLF1IP1 antibodyPBIP1 antibodyPolo-box-interacting protein 1 antibody

  see all

Anti-MLF1 Interacting Protein/PBIP1 (phospho T78) antibody images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLF1 Interacting Protein/PBIP1 (phospho T78) antibody (ab34911)

  ab34911 at 20 µg/ml staining mitotic cells in colon adenocarcinoma at 60X. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.