Overview
- Product nameMMP1 Human ELISA KitSee all MMP1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture extracts, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 8 pg/ml
- Range24.69 pg/ml - 18000 pg/ml
- Recovery
92 %
Sample specific recovery Sample type Average % Range Cell culture extracts 91.47 78% - 103% Tissue Extracts 92.68 79% - 104% - Species reactivityReacts with: Human
- Product overview
ab100604 Human MMP1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP1 cell lysate and tissue lysate. This assay employs an antibody specific for human MMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MMP1 antibody is added. After washing away unbound biotinylated antibody, HRP- conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 2X Cell Lysate Buffer Item J 1 x 5ml 440X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent 5x concentrated buffer. For Detection Antibody and HRP-Streptavidin concentrate diluent. 1 x 15ml Detection Antibody MMP-1: biotinylated anti-human MMP-1 2 vials MMP 1 Standard Human(recombinant) 2 vials MMP 10 Microplate (12 strips x 8 wells) coated with anti-human MMP-10 1 unit Sample Diluent Buffer 5x concentrated buffer For Standard/Sample (cell lysate/tissue lysate) diluent. 1 x 10ml Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml -
Research Areas
Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsUndergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Target information above from: UniProt accession
P03956
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 27 kDa interstitial collagenaseCLGCLGN
- Fibroblast collagenaseInterstitial collagenase Matrix metallopeptidase 1 (interstitial collagenase)Matrix Metalloproteinase 1 Matrix metalloproteinase-1MMP 1 MMP-1MMP1MMP1_HUMAN
see all
- Entrez Gene: 4312 Human
- SwissProt: P03956 Human
- Unigene: 83169 Human
Applications
Our Abpromise guarantee covers the use of ab100604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
MMP1 Human ELISA Kit images
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ELISA - Human MMP-1 ELISA Kit (ab100604)
Protocols
References for MMP1 Human ELISA Kit (ab100604)
ab100604 has not yet been referenced specifically in any publications.
