MMP1 Human ELISA Kit (ab100603)
- Product nameMMP1 Human ELISA KitSee all MMP1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 8 pg/ml
- Range24.69 pg/ml - 18000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.66 90% - 106% Serum 97.78 88% - 106% Plasma 99.59 90% - 107%
- Species reactivityReacts with: Human
- Product overview
Abcam’s MMP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for the quantitative measurement of Human MMP1 pro and active forms in serum and plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended) and cell culture supernatants.
This assay employs an antibody specific for Human MMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 440X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Human MMP-1 Antibody 2 vials MMP 1 Microplate 1 unit MMP 1 Standard Human(recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsUndergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
- 27 kDa interstitial collagenaseCLGCLGN
- Fibroblast collagenaseInterstitial collagenase Matrix metallopeptidase 1 (interstitial collagenase)Matrix Metalloproteinase 1 Matrix metalloproteinase-1MMP 1 MMP-1MMP1MMP1_HUMAN
Our Abpromise guarantee covers the use of ab100603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
MMP1 Human ELISA Kit images
TYPICAL STANDARD CURVE – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
References for MMP1 Human ELISA Kit (ab100603)
ab100603 has not yet been referenced specifically in any publications.