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Overview

  • Product nameMMP1 Human ELISA KitSee all MMP1 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 8 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.66 90% - 106%
    Serum 97.78 88% - 106%
    Plasma 99.59 90% - 107%

  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MMP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for the quantitative measurement of Human MMP1 pro and active forms in serum and plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended) and cell culture supernatants.

    This assay employs an antibody specific for Human MMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples

  • Tested applicationsELISA more details

Properties

  • FunctionCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P03956 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        27 kDa interstitial collagenaseCLGCLGN
        Fibroblast collagenaseInterstitial collagenase Matrix metallopeptidase 1 (interstitial collagenase)Matrix Metalloproteinase 1 Matrix metalloproteinase-1MMP 1 MMP-1MMP1MMP1_HUMAN
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100603 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      MMP1 Human ELISA Kit images

      • ELISA - MMP1 Human ELISA Kit (ab100603)
        ELISA - MMP1 Human ELISA Kit (ab100603)
        TYPICAL STANDARD CURVE – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

      Protocols

      References for MMP1 Human ELISA Kit (ab100603)

      ab100603 has not yet been referenced specifically in any publications.

      Publishing research using ab100603 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100603 Submit a Question for ab100603

      Displaying 1 - 3 of 3 results for Abreviews and Q&A

      10
      Votes: Highest First

      Hello
      I would like to know the date of manufacture and when the antibodies
      below expire.
      AB100603
      Lote GR47531-1
      AB100651
      Lote GR49880-1
      Thanks and best regards,

      Thank you for your inquiry.

      For ab100603 lot GR47531-1, it was entered into stock in June 2011. We set an expiration guidelinefor 1 year from that point, so June 2012.

      For ab100651 lot GR49880-1 was put into stock in July, Aug and Oct ...

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      no signal. Our customer purchased ab100603 (5kits). He tested MMP-1 ELISA although he found precipitation in assay diluents. As a result he has used 2kits with different lot# but all not detected standards. Please check this.

      Thank you and your customerfor taking the time to complete our questionnaire and contact us. I am sorry to hear your customerhas had difficulty obtaining satisfactory results from this antibody.

      The details your customerhas kindly provided will...

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      I would like to have more information concerning the MMP elisa kits. There are elisa kits available for MMP-1 (ab100603), MMP-3 (ab100607) and MMP-13 (ab100605).

      These kits cross-react with human MMP-1, MMP-3 and MMP-13. We would like to order ...

      Read More

      Thank you for contacting Abcam.

      Unfortunately, to our knowledge ab100603, ab100607, and ab100605 have not been tested with monkey samples so there is no cross reactivity data available. However, I believe that MMPs are very well conserved among ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"