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Anti-MMP1 antibody [EP1247Y] (ab52631)

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2 questions for ab52631

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Question 1

Thursday 28-July-2011

I have ordered the following antibodies, please can you let me know what to dilute them down in ie either PBS or distilled water? Thanking you in advance.Ab 66157- MMP12Ab 51708- IL20Ab 53015 MMP3Ab 58803 MMP9Ab 52631 MMP1  Best wishes 

ANSWER:

  

Thank you for contacting us.

We would suggest using PBS with 1% BSA as a diluting buffer.

Please be advised that if you would like to store these antibodies at -20 or as recommended on the datasheet then we would recommend storing the antibodies without diluting these. We recommend aliquoting the antibodies in small volumes before storage and using the aliquots as required.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Question 2

Friday 26-February-2010

I am currently a science student and looking for MMP1 antibody. I had a few questions with regards to the ab52631 antibody. 1. Whether it cross reacts with other MMP's and if you have checked for that? 2. does it recognize both the active and latent form of MMP1? 3. Whether the other applications you have indicated have been tested? Thanks

ANSWER:

  

After investigation with the laboratory, I can confirm we have not extensively tested this antibody against all other MMP family members, this antibody, along with the other MMP antibodies should be specific for its particular target. Also, ab52631 should detect both forms of the protein. It detects a region after the cleave site.

Having reviewed our datasheet, I can confirm ab52631 has only been tested in Flow Cytometry, ICC, IHC-P, IP and WB.

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