MMP1 antibody [EP1247Y] (ab52631)

Product Name 

MMP1 antibody [EP1247Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1247Y] to MMP1

Immunogen 

Synthetic peptide located between 115 and 140 residues in MMP1 (Human)

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Tested applications 
(see key)

Flow Cyt, ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/20.
ICC: 1/100 - 1/250.
IHC-P: 1/50 - 1/100. Antigen retrieval step strongly recommended for enhanced signal.
IP: 1/30.
WB: 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

SKBr-3 cell lysate.
Human cervical carcinoma.

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.
MMP1, also known as interstitial collagenase, is the only enzyme that is able to initiate the breakdown of the interstitial collagens, types I, II, and III.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1247Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

Produced under U.S. Patent No. 5,675,063.

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MMP1 antibody [EP1247Y] images:

References for MMP1 antibody [EP1247Y] (ab52631)

  This product has been used in:

Shen LC et al. Expression of osteonectin/secreted protein acidic and rich in cysteine and matrix metalloproteinases in ameloblastoma. J Oral Pathol Med : (2010). IHC-P; Human. PubMed: 20070483

If you publish research using ab52631 please let us know so that we can cite the reference on this datasheet.

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