Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Dear technical team, Our customer would like to confirm that ab38929 detects both pro- and active-MMP1. If this antibody detects both form, IHC stained results also both pro- and active-MMP1? Check this for our customer. Thank you. Best regards, |
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ANSWER: |
Thank you for your enquiry. I can confirm that ab38929 MMP1 antibody is known to detect both pro and active forms of MMP1. I hope this is helpful to you. Should you have any further questions, please do not hesitate to contact us. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-MMP1 antibody - Hemopexin domain (ab38929)
Lane 1 : Human MMP1.
Lane 2 : Cell Media from human chondrosarcoma (no treatment).
Lane 3 : Cell Media from human chondrosarcoma (treated with TPA).
Predicted band size : 54 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
ab47116 (2µg/ml) staining Zinc Alpha 2 Glycoprotein in human breast tumour using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining in Myoepithelial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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