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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP1 antibody - Hemopexin domain
See all MMP1 products (25) ...
Description
Rabbit polyclonal to MMP1 - Hemopexin domain
Specificity
This antibody binds to MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9).
Tested applications
IHC-P, ICC/IF, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Horse, Dog, Human
Immunogen
Synthetic peptide based on the hemopexin domain of the human MMP1 sequence. (Peptide available as ab41003.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution. (PubMed: 18434409)
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.(When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection.)
Target
Function
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP03956
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CLGN antibody
- 27 kDa interstitial collagenase antibody
- CLG antibody
see all
Database links
- Entrez Gene: 4312 Human
- Omim: 120353 Human
- SwissProt: Q9XSZ5 Horse
- SwissProt: P03956 Human
- Unigene: 83169 Human
Anti-MMP1 antibody - Hemopexin domain images:
Western blot - MMP1 antibody (ab38929)
All lanes : Anti-MMP1 antibody - Hemopexin domain (ab38929)
Lane 1 : Human MMP1.
Lane 2 : Cell Media from human chondrosarcoma (no treatment).
Lane 3 : Cell Media from human chondrosarcoma (treated with TPA).
Predicted band size : 54 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP1 antibody - Hemopexin domain (ab38929)
ab47116 (2µg/ml) staining Zinc Alpha 2 Glycoprotein in human breast tumour using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining in Myoepithelial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
References for Anti-MMP1 antibody - Hemopexin domain (ab38929)
This product has been referenced in:
- Keld Ret al. The ERK MAP kinase-PEA3/ETV4-MMP-1 axis is operative in oesophageal adenocarcinoma. Mol Cancer 9:313 (2010). WB; Human.Read more (PubMed: 21143918) »
- Barbaresi Set al. Effects of human papillomavirus type 16 E5 deletion mutants on epithelial morphology: functional characterization of each transmembrane domain. J Gen Virol 91:521-30 (2010). IHC-P; Human.Read more (PubMed: 19812262) »
See all 6 publications for this product
Publishing research using ab38929? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"