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Standard curve for MMP1 (Analyte: ab39297); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [SB12e] to MMP1 - BSA and Azide free (ab25483) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP1 - Propeptide domain (ab38924) at 0.5µg/ml.
All lanes : Anti-MMP1 antibody - Propeptide domain (ab38924) at 1/1000 dilution
Lane 1 : Human MMP 1
Lane 2 : Cell media from Human chondrosarcoma (no treatment)
Lane 3 : Cell media from Human chondrosarcoma (treated with TPA)
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 51-53 kDa (why is the actual band size different from the predicted?)
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