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MMP1 protein (Human) (ab39297)

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Overview

Product name

MMP1 protein (Human)
See all MMP1 products (7) ...

Protein description

Full length native protein (purified) of Human MMP1. The enzyme is supplied as a mixture of the zymogen and processed enzyme.

Expression host

Native

Properties

Purification notes

This enzyme was purified from stimulated human fibroblasts, and is essentially free of contaminating MMPs or TIMPs. The enzyme is supplied as a mixture of the zymogen and active enzyme.

Form

Liquid

Storage instructions

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, 0.025% Brij 35, 0.175M Sodium chloride, 5mM Calcium chloride, 10 mM MES, pH 5.5

Concentration

Concentration information loading...

Research areas

Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

  • Sandwich ELISA - MMP1 protein (Human) (ab39297)Sandwich ELISA - MMP1 protein (Human) (ab39297) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab39297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

  • SDS-PAGE

     

  • ELISA

     

Application notes

ELISA: Use at an assay dependent dilution.
SDS-PAGE (as a Control/standard): Use at an assay dependent dilution.
WB (as a Control/standard): Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protein info

Function

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

Sequence similarities

Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.

Domain

There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational
modifications

Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionP03956 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

MMP1 protein (Human) images:

  Sandwich ELISA - MMP1 protein (Human) (ab39297)

Sandwich ELISA - MMP1 protein (Human) (ab39297)

Standard curve for MMP1 (Analyte: ab39297); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [SB12e] to MMP1 - BSA and Azide free (ab25483) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP1 - Propeptide domain (ab38924) at 0.5µg/ml.

References for MMP1 protein (Human) (ab39297)

ab39297 has not yet been referenced specifically in any publications.

Publishing research using ab39297? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"