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Overview

  • Product nameMMP1 protein (Human)See all MMP1 proteins and peptides ...
  • Protein descriptionFull length Human MMP1 protein (purified from stimulated human fibroblasts).
  • Expression hostNative

    • Properties

  • Purification notesPurified from stimulated human fibroblasts, and is free of contaminating MMPs or TIMPs. The enzyme is supplied as a mixture of the zymogen and processed enzyme.
  • Biological activityab39301 is a true collagenase, and along with MMP8 and MMP13 can cleave all three strands of intact native collagen. Unlike MMP2 (Type IV Collagenase, Gelatinase A), MMP1 is not usually constitutively produced. When MMP1 production is stimulated, native MMP inhibitors (TIMPs) usually follow to quench them.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10 mM MES, 50% Glycerol, 0.025% Brij 35, 5mM Calcium chloride, 5mM EDTA, 175mM Sodium chloride, pH 5.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab39301 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
    SDS-PAGE
  • Application notesSDS-PAGE Standard: Use at an assay dependent dilution.
    WB Standard: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        27 kDa interstitial collagenaseCLGCLGN
        CLGNCLGNcollagenase, fibroblastcollagenase, interstitialFibroblast collagenaseFibroblast collagenaseInterstitial collagenaseInterstitial collagenase precursorInterstitial collagenase precursorMatrix metallopeptidase 1 (interstitial collagenase)Matrix metalloprotease 1Matrix Metalloproteinase 1Matrix Metalloproteinase 1Matrix metalloproteinase-1MM01MM01MMP 1MMP 1MMP-1MMP1MMP1_HUMANOTTHUMP00000045866
      see all
  • FunctionCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P03956 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for MMP1 protein (Human) (ab39301)

    ab39301 has not yet been referenced specifically in any publications.

    Publishing research using ab39301 ? Please let us know so that we can cite the reference in this datasheet

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    I want to use this product in cell culture to stimulate my cells. Is this protein active and able to use this way? Does it require another product for activation?

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    The MMP1 protein ab39301 contains EDTA to stabilize the protein, and has no enzymatic activity. In theory activity can be achieved by dialysis or gel filtration and adding zinc and calcium, but with low yield.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"