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Overview

  • Product nameMMP10 Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 3 pg/ml
  • Range
    2.06 pg/ml - 1500 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.31 87% - 109%
    Serum 96.54 85% - 108%
    Plasma 94.57 84% - 105%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's MMP10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP10 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended) and cell culture supernates.

     

    This assay employs an antibody specific for human MMP10 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MMP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionCan degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P09238 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        AV377895Matrix Metalloproteinase 10Matrix metalloproteinase-10
        MMP 10 MMP-10Mmp10MMP10_HUMANSL 2SL-2SL2STMY2Stromelysin 2 Stromelysin II Stromelysin-2Stromelysin2Transin 2Transin-2Transin2
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100602 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      MMP10 Human ELISA Kit images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab100602.

      Protocols

      References for MMP10 Human ELISA Kit (ab100602)

      ab100602 has not yet been referenced specifically in any publications.

      Publishing research using ab100602 ? Please let us know so that we can cite the reference in this datasheet

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      I would appreciate it if you could send me information regarding your MMP10 Human ELISA Kit (ref. ab100602). Specifically, I need to know which forms of MMP-10 are recognized by the kit. MMP-10 is secreted as proenzyme (proMMP10) and it can be proteolytic...

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      Thank you for contacting Abcam regarding ab100602. I can confirm that this kit will recognize both pro and active forms of MMP10 protein. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"