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Immunocytochemistry/ Immunofluorescence - MMP10 antibody [5E4] (ab49473)
![Immunocytochemistry/ Immunofluorescence - MMP10 antibody [5E4] (ab49473)](/ps/datasheet/images/49/ab49473/MMP10-Primary-antibodies-ab49473-1.jpg)
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Product Name
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MMP10 antibody [5E4]
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See all MMP10 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [5E4] to MMP10
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Immunogen
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Prokaryotic recombinant fusion protein corresponding to amino acids 342-476 of the human MMP10 molecule.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/25 - 1/50. Heat mediated antigen retrieval is recommended before commencing with IHC staining protocol.
ICC/IF: Use at a concentration of 1 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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IHC-P: Ulcerative colitis tissue.
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Cellular localization
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Secreted
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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Proteins of the matrix metalloproteinase (MMP) family are zinc containing proteolytic enzymes involved in the breakdown of the extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling. They also play an important role in apoptosis, tumor cell growth, invasion, metastasis, as well as in angiogenesis and wound healing. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.
Most of the MMP's contain a common domain structure, which include a signal sequence, a propeptide, a catalytic domain and a hemopexin-like (Hpx) domain.
MMP10 degrades proteoglycans, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. and fibronectin. It activates procollagenase.
The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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5E4
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Myeloma
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P3-NS1/1-Ag4-1
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Isotype
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IgG1
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP10 antibody [5E4] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP10 antibody [5E4] images, references, products related to ab49473 and other tools.
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MMP10 antibody [5E4] images:
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 Immunocytochemistry/ Immunofluorescence - MMP10 antibody [5E4] (ab49473)
ICC/IF image of ab49473 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49473, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for MMP10 antibody [5E4] (ab49473)
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This clone has been used in:
Gill JH et al. MMP-10 is overexpressed, proteolytically active, and a potential target for therapeutic intervention in human lung carcinomas. Neoplasia 6:777-85 (2004). PubMed: 15720804
If you publish research using ab49473 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MMP10
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MMP10 antibody [5E4] - more information
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Customer reviews (feedback) regarding MMP10 antibody [5E4] |
Customer FAQs regarding MMP10 antibody [5E4] |
Protocols for MMP10 antibody [5E4] |
Price and availability of products related to MMP10 antibody [5E4] |
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MMP10 antibody [5E4] - related products:
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MMP10 antibody [5E4] - other tools:
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Contact Abcam with a Technical Enquiry about MMP10 antibody [5E4] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - MMP10 antibody [5E4] (ab49473)](/index.html?datasheet=49473#MMP10-Primary-antibodies-ab49473-1.jpg)
