![Immunocytochemistry/ Immunofluorescence - MMP10 antibody [5E4] (ab49473) image](#MMP10-Primary-antibodies-ab49473-1.jpg)
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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP10 antibody [5E4]
See all MMP10 products (9) ...
Description
Mouse monoclonal [5E4] to MMP10
Tested applications
IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Prokaryotic recombinant fusion protein corresponding to amino acids 342-476 of the human MMP10 molecule.
Positive control
IHC-P: Ulcerative colitis tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
5E4
Myeloma
P3-NS1/1-Ag4-1
Isotype
IgG1
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Immunocytochemistry/ Immunofluorescence - MMP10 antibody [5E4] (ab49473)
![Immunocytochemistry/ Immunofluorescence - MMP10 antibody [5E4] (ab49473) image](/ps/datasheet/images/49/ab49473/MMP10-Primary-antibodies-ab49473-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab49473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
- 1 Image
ICC/IF
Application notes
IHC-P: 1/25 - 1/50. Heat mediated antigen retrieval is recommended before commencing with IHC staining protocol.
ICC/IF: Use at a concentration of 1 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP09238
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix metalloproteinase 10 antibody
- Matrix metalloproteinase-10 antibody
- MMP 10 antibody
see all
Database links
- Entrez Gene: 4319 Human
- Omim: 185260 Human
- SwissProt: P09238 Human
- Unigene: 2258 Human
Anti-MMP10 antibody [5E4] images:
Immunocytochemistry/ Immunofluorescence - MMP10 antibody [5E4] (ab49473)
ICC/IF image of ab49473 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49473, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MMP10 antibody [5E4] (ab49473)
This product has been referenced in:
- Gill JHet al. MMP-10 is overexpressed, proteolytically active, and a potential target for therapeutic intervention in human lung carcinomas. Neoplasia 6:777-85 (2004).Read more (PubMed: 15720804) »
See 1 publication for this product
Publishing research using ab49473? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"