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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP10 antibody - Hemopexin domain
See all MMP10 products (9) ...
Description
Rabbit polyclonal to MMP10 - Hemopexin domain
Specificity
This antibody is specific for MMP10, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc).
Tested applications
Cross reactivity
Reacts with
Dog, Human
Immunogen
Synthetic peptide based on the Hemopexin domain of the human MMP10.
(Peptide available as ab41076.)
Epitope
This antibody binds to the Hemopexin domain near the carboxyterminus.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP10 antibody - Hemopexin domain (ab28206)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 - 1/5000. Detects a band of approximately 56 kDa (pro-form, with a predicted molecular weight: 54 kDa) and a 46kDa band (inital active form), using reducing conditions. The final, hemopexin cleaved form is not detected. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP09238
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix Metalloproteinase 10 antibody
- Matrix Metalloproteinase 10 antibody
- Matrix metalloproteinase-10 antibody
see all
Database links
- Entrez Gene: 4319 Human
- Omim: 185260 Human
- SwissProt: P09238 Human
- Unigene: 2258 Human
Anti-MMP10 antibody - Hemopexin domain images:
Western blot - MMP10 antibody - Hemopexin domain (ab28206)
All lanes : Anti-MMP10 antibody - Hemopexin domain (ab28206) at 1/1000 dilution
Lane 1 : MMP-10 at 0.1 µg
Lane 2 : MMP-10 at 0.01 µg
Lane 3 : MMP-10 at 0.001 µg
Lane 4 : MMP-10 at 0.1 µg
Lane 5 : MMP-10 at 0.01 µg
Lane 6 : MMP-10 at 0.001 µg
Predicted band size : 54 kDa
Observed band size : 46,56 kDa (why is the actual band size different from the predicted?)
Lanes 1-3 = pro-enzyme
Lanes 4-6 = activated form
The 46KDa band represents the initial active forms and the 56KDa band represents the pro-form.
Protocols
References for Anti-MMP10 antibody - Hemopexin domain (ab28206)
This product has been referenced in:
- Doehn Uet al. RSK is a principal effector of the RAS-ERK pathway for eliciting a coordinate promotile/invasive gene program and phenotype in epithelial cells. Mol Cell 35:511-22 (2009). WB; Human, Dog.Read more (PubMed: 19716794) »
See 1 publication for this product
Publishing research using ab28206? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"