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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP10 antibody - Propeptide domain
See all MMP10 products (9) ...
Description
Rabbit polyclonal to MMP10 - Propeptide domain
Specificity
This antibody is specific for MMP10 and MMP3, but does not cross react with the other MMP family members (MMP1, MMP2, MMP9, etc.).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the propeptide domain of the human MMP10.
(Peptide available as ab41074.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP10 antibody - Propeptide domain (ab28205)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, 1/5000 for chemiluminescent substrates. Detects a band of approximately 56 kDa (predicted molecular weight: 54 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP09238
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix Metalloproteinase 10 antibody
- Matrix Metalloproteinase 10 antibody
- Matrix metalloproteinase-10 antibody
see all
Database links
- Entrez Gene: 4319 Human
- Omim: 185260 Human
- SwissProt: P09238 Human
- Unigene: 2258 Human
Anti-MMP10 antibody - Propeptide domain images:
Western blot - MMP10 antibody - Propeptide domain (ab28205)
All lanes : Anti-MMP10 antibody - Propeptide domain (ab28205) at 1/1000 dilution
Lane 1 : MMP10 (proenzyme) at 0.1 µg
Lane 2 : MMP10 (proenzyme) at 0.01 µg
Lane 3 : MMP10 (proenzyme) at 0.001 µg
Lane 4 : MMP10 (active) at 0.1 µg
Lane 5 : MMP10 (active) at 0.01 µg
Lane 6 : MMP10 (active) at 0.001 µg
Predicted band size : 54 kDa
Observed band size : 42,56 kDa (why is the actual band size different from the predicted?)
There are no apparent bands in lanes 4-6 because this is the active form of MMP10, where the propeptide domain has been cleaved and this antibody is specific to the propeptide domain.
Protocols
References for Anti-MMP10 antibody - Propeptide domain (ab28205)
This product has been referenced in:
- Miller JPet al. Matrix Metalloproteinases Are Modifiers of Huntingtin Proteolysis and Toxicity in Huntington's Disease. Neuron 67:199-212 (2010). WB, ICC/IF; Human.Read more (PubMed: 20670829) »
See 1 publication for this product
Publishing research using ab28205? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"