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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP10 antibody
See all MMP10 products (9) ...
Description
Rabbit polyclonal to MMP10
Specificity
Detects endogenous levels of total MMP10 protein.
Tested applications
ICC/IF, ELISA, Flow Cyt, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) from an internal region of MMP10.
Positive control
HepG2 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Immunocytochemistry/ Immunofluorescence - MMP10 antibody (ab59437)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab59437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500 - 1/1000.
ELISA
ELISA: 1/10000ELISA: 1/10000
Flow Cyt: Use at an assay dependent dilution. (PubMed: 21133852)
IHC-Fr: Use at an assay dependent dilution. (PubMed: 21133852)
Target
Function
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP09238
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix metalloproteinase 10 antibody
- Matrix metalloproteinase-10 antibody
- MMP 10 antibody
see all
Database links
- Entrez Gene: 4319 Human
- Entrez Gene: 17384 Mouse
- Entrez Gene: 117061 Rat
- Omim: 185260 Human
- SwissProt: P09238 Human
- SwissProt: O55123 Mouse
- SwissProt: P07152 Rat
- Unigene: 2258 Human
see all
Anti-MMP10 antibody images:
Immunocytochemistry/ Immunofluorescence - MMP10 antibody (ab59437)
Immunofluorescent analysis of MMP10 in HepG2 cells with ab59437 (1/500 dilution); in the presence or absence of immunizing peptide.
References for Anti-MMP10 antibody (ab59437)
This product has been referenced in:
- Clark RTet al. T cell production of matrix metalloproteases and inhibition of parasite clearance by TIMP-1 during chronic Toxoplasma infection in the brain. ASN Neuro : (2010). Flow Cyt, IHC-Fr; Mouse.Read more (PubMed: 21133852) »
See 1 publication for this product
Publishing research using ab59437? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"