MMP11 antibody [EP1259Y] (ab52904)

Product Name 

MMP11 antibody [EP1259Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1259Y] to MMP11

Immunogen 

Synthetic peptide corresponding to residues near the C-terminus of human MMP11.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. We strongly recommend customers perform an antigen retrieval step.
WB: 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Is unsuitable for Flow Cyt or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MCF-7 cell lysate, human breast carcinoma tissue or MCF7 cells.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1 proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix.

MMP11, also known as stromelysin 3, is overexpressed in the stromal cells of invasive breast carcinomas but not in the stromal cells surrounding benign breast fibroadenomas. MMP11 is secreted in its proenzyme from and the zymogen has a mass of 64 kDa. The enzyme is rapidly activated to a 58 kDa form, a 45-47 kDa size, then to a 28 kDa final active form. MMP11 is not constitutively produced by most cell types. It has been found in lung fibroblasts and gingival fibroblasts when stimulated with EGF, as well as breast tumor and osteocarcinoma cell lines.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1259Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

Produced under U.S. Patent No. 5,675,063.

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