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Anti-MMP11 antibody [EP1259Y] (ab52904)

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Overview

Product name

Anti-MMP11 antibody [EP1259Y]
See all MMP11 products (10) ...

Description

Rabbit monoclonal [EP1259Y] to MMP11

Tested applications

ICC/IF, WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues near the C-terminus of human MMP11.

Positive control

MCF-7 cell lysate, human breast carcinoma tissue or MCF7 cells.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1259Y

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

  • Western blot - MMP11 antibody [EP1259Y] (ab52904)Western blot - MMP11 antibody [EP1259Y] (ab52904) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - MMP11 antibody [EP1259Y] (ab52904)Immunohistochemistry (Paraffin-embedded sections) - MMP11 antibody [EP1259Y] (ab52904) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - MMP11 antibody [EP1259Y] (ab52904)Immunocytochemistry/ Immunofluorescence - MMP11 antibody [EP1259Y] (ab52904) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    ICC/IF

     ICC/IF: 1/100 - 1/250.

    ICC/IF: 1/100 - 1/250.

  • ShowHide1 Image

    WB

     WB: 1/500Detects a band ...Read more →

    WB: 1/500Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.(An...Read more →

    IHC-P: 1/100 - 1/250.(Antigen retrieval is recommended.)

Application notes

Is unsuitable for ,Flow Cyt or IP.

Target

Function

May play an important role in the progression of epithelial malignancies.

Tissue specificity

Specifically expressed in stromal cells of breast carcinomas.

Sequence similarities

Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational
modifications

The precursor is cleaved by a furin endopeptidase.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionP24347 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Matrix Metalloproteinase 11 antibody
  • Matrix metalloproteinase-11 antibody
  • MMP-11 antibody
  • Mmp11 antibody
  • MMP11_HUMAN antibody
  • SL 3 antibody
  • SL-3 antibody
  • SL3 antibody
  • ST3 antibody
  • STMY3 antibody
  • Stromelysin 3 antibody
  • Stromelysin III antibody
  • Stromelysin-3 antibody
see all

Database links

see all

Anti-MMP11 antibody [EP1259Y] images:

  Western blot - MMP11 antibody [EP1259Y] (ab52904)

Western blot - MMP11 antibody [EP1259Y] (ab52904)

Anti-MMP11 antibody [EP1259Y] (ab52904) at 1/500 dilution + MCF-7 cell lysate at 10 µg

Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution

Predicted band size : 55 kDa
Observed band size : 55 kDa

  Immunohistochemistry (Paraffin-embedded sections) - MMP11 antibody [EP1259Y] (ab52904)

Immunohistochemistry (Paraffin-embedded sections) - MMP11 antibody [EP1259Y] (ab52904)

Ab52904 (1:100) staining human MMP11 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

  Immunocytochemistry/ Immunofluorescence - MMP11 antibody [EP1259Y] (ab52904)

Immunocytochemistry/ Immunofluorescence - MMP11 antibody [EP1259Y] (ab52904)

Immunofluorescent staining of MCF7 cells using ab52904 (1:100).

References for Anti-MMP11 antibody [EP1259Y] (ab52904)

ab52904 has not yet been referenced specifically in any publications.

Publishing research using ab52904? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"