Anti-MMP11 antibody (ab119284)

Overview

• Product nameAnti-MMP11 antibodySee all MMP11 primary antibodies ...
• Description
  Rabbit polyclonal to MMP11
• Tested applicationsWB, IHC-P, ICC/IF, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic peptide conjugated to KLH, derived from internal sequence amino acids of Human MMP11.

• Positive controlHuman placenta and spleen tissues; A549 cell lysate; A549 cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  Note: PBS without Mg2+, Ca2+
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • Angiogenesis
  • ECM enzymes
  • MMPs

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • TIMPs

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Enzymes
  • MMP

  • Cardiovascular
  • Angiogenesis
  • Adhesion / ECM
  • Matrix Metalloproteinases
  • MMP

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Metalloprotease
  • MMPs

Applications

Target

• FunctionMay play an important role in the progression of epithelial malignancies.
• Tissue specificitySpecifically expressed in stromal cells of breast carcinomas.
• Sequence similaritiesBelongs to the peptidase M10A family.
  Contains 4 hemopexin-like domains.
• DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
• Post-translational
  modificationsThe precursor is cleaved by a furin endopeptidase.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P24347 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4320 Human
  • Entrez Gene: 17385 Mouse
  • Entrez Gene: 25481 Rat
  • Omim: 185261 Human
  • SwissProt: P24347 Human
  • SwissProt: Q02853 Mouse
  • SwissProt: Q499S5 Rat
  • Unigene: 143751 Human

  • Unigene: 4561 Mouse
  • Unigene: 11123 Rat

  see all

• Alternative names
  Matrix Metalloproteinase 11 antibodyMatrix metalloproteinase-11 antibodyMMP-11 antibody

  Mmp11 antibodyMMP11_HUMAN antibodySL 3 antibodySL-3 antibodySL3 antibodyST3 antibodySTMY3 antibodyStromelysin 3 antibodyStromelysin III antibodyStromelysin-3 antibody

  see all

Anti-MMP11 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP11 antibody (ab119284)
  ab119284, at 10 µg/ml, staining MMP11 in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP11 antibody (ab119284)
  ab119284, at 10 µg/ml, staining MMP11 in Formalin-fixed, Paraffin-embedded Human spleen tissue by Immunohistochemistry.
• 

  Western blot - Anti-MMP11 antibody (ab119284)
  All lanes : Anti-MMP11 antibody (ab119284) at 1/500 dilution
  
  Lane 1 : A549 cell lysate
  Lane 2 : A549 cell lysate with synthesized peptide
  
  
  Predicted band size : 55 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-MMP11 antibody (ab119284)
  ab119284, at 1/100, staining MMP11 in A549 cells by Immunofluorescence. The image on the right shows cells treated with synthesised peptide.