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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP12 antibody - Carboxyterminal end
See all MMP12 products (12) ...
Description
Rabbit polyclonal to MMP12 - Carboxyterminal end
Specificity
The antibody binds to MMP12, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the carboxyterminal end of human MMP12.
(Peptide available as ab41086.)
Positive control
Cell media from human embryonal rhabdomyosarcoma treated with TNF-alpha.
General notes
Stimulated macrophages produce MMP-12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of MMP-12 produced.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP12 antibody (ab38910)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for non human samples. When used against the reduced protein, the antibody identifies bands at 54 kDa, 45 kDa, and activated forms in between (predicted molecular weight: 54 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Tissue specificity
Found in alveolar macrophages but not in peripheral blood monocytes.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP39900
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.4.24.65 antibody
- HME antibody
- Macrophage elastase antibody
see all
Database links
- Entrez Gene: 4321 Human
- Omim: 601046 Human
- SwissProt: P39900 Human
- Unigene: 1695 Human
- Unigene: 709832 Human
Anti-MMP12 antibody - Carboxyterminal end images:
Western blot - MMP12 antibody (ab38910)
Anti-MMP12 antibody - Carboxyterminal end (ab38910) + Cell media from human embryonal rhabdomyosarcoma (treated with TNF alpha)
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 45 kDa,66 kDa. We are unsure as to the identity of these extra bands.
When used against the reduced protein, the antibody identifies bands at 54 kDa, 45 kDa, and activated forms in between.
Protocols
References for Anti-MMP12 antibody - Carboxyterminal end (ab38910)
This product has been referenced in:
- LaPan Pet al. Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum. BMC Pulm Med 10:40 (2010). ELISA; Human.Read more (PubMed: 20678199) »
See 1 publication for this product
Publishing research using ab38910? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"