Anti-MMP12 antibody [EP1261Y] - Carboxyterminal end (ab52897)

  Western blot - MMP12 antibody [EP1261Y] - Carboxyterminal end (ab52897)

Predicted band size : 54 kDa


Based on Western blot, ab52897 recognizes both pro and active MMP. The propeptide is 54 kDa, and 45 kDa and 22 kDa are active forms. The primary was used at 1:100 to detect recombinant MMP12 protein, but can be diluted more to decrease the intensity of the bands.

  Immunohistochemistry (Paraffin-embedded sections) - MMP12 antibody [EP1261Y] (ab52897)

Ab52897 (1:100) staining human MMP12 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

  Immunocytochemistry/ Immunofluorescence - MMP12 antibody [EP1261Y] (ab52897)

Immunofluorescent staining of HeLa cells using ab52897 (1:100).

  Flow Cytometry - MMP12 antibody [EP1261Y] - Carboxyterminal end (ab52897)

Overlay histogram showing A549 cells stained with ab52897 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52897, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.