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- Proteins and Peptides
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Product name
Anti-MMP12 antibody - Hinge region
See all MMP12 products (14) ...
Description
Rabbit polyclonal to MMP12 - Hinge region
Specificity
ab38935 does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc). ab38935 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the hinge region of Human MMP12.
(Peptide available as ab440 21.)
Positive control
Cell media from human metastatic melanoma (treated with TNF alpha).
General notes
Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000. Detects a band of approximately 54, 45 , 22 kDa.
ICC/IF: Use a concentration of 5 µg/ml.
Target
Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Tissue specificity
Found in alveolar macrophages but not in peripheral blood monocytes.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP39900
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.4.24.65 antibody
- HME antibody
- Macrophage elastase antibody
see all
Database links
- Entrez Gene: 4321 Human
- Omim: 601046 Human
- SwissProt: P39900 Human
- Unigene: 1695 Human
Anti-MMP12 antibody - Hinge region images:
Western blot - MMP12 antibody (ab38935)
Anti-MMP12 antibody - Hinge region (ab38935) + Cell media from human metastatic melanoma (treated with TNF alpha)
Observed band size : 45,54 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-MMP12 antibody - Hinge region(ab38935)
ICC/IF image of ab38935 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38935, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-MMP12 antibody - Hinge region (ab38935)
This product has been referenced in:
- Grossetete M et al. Elevation of matrix metalloproteinases 3 and 9 in cerebrospinal fluid and blood in patients with severe traumatic brain injury. Neurosurgery 65:702-8 (2009). WB; Human.Read more (PubMed: 19834375) »
See 1 publication for this product
Publishing research using ab38935? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"