![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP12 antibody [MM0025-7G20] (ab56305) image](#MMP12-Primary-antibodies-ab56305-1.jpg)
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Product name
Anti-MMP12 antibody [MM0025-7G20]
See all MMP12 products (14) ...
Description
Mouse monoclonal [MM0025-7G20] to MMP12
Specificity
This antibody recognizes human MMP12 in western blot without cross reactivity with other human MMPs.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human MMP12
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Clonality
Monoclonal
Clone number
MM0025-7G20
Isotype
IgG2
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP12 antibody [MM0025-7G20] (ab56305)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP12 antibody [MM0025-7G20] (ab56305) image](/ps/datasheet/images/56/ab56305/MMP12-Primary-antibodies-ab56305-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab56305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/300.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.
Target
Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Tissue specificity
Found in alveolar macrophages but not in peripheral blood monocytes.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP39900
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.4.24.65 antibody
- HME antibody
- Macrophage elastase antibody
see all
Database links
- Entrez Gene: 4321 Human
- Omim: 601046 Human
- SwissProt: P39900 Human
- Unigene: 1695 Human
Anti-MMP12 antibody [MM0025-7G20] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP12 antibody [MM0025-7G20] (ab56305)
ab56305 at 1/300 dilution, staining MMP12 in human placenta tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
References for Anti-MMP12 antibody [MM0025-7G20] (ab56305)
ab56305 has not yet been referenced specifically in any publications.
Publishing research using ab56305? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"