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Overview

  • Product nameAnti-MMP12 antibodySee all MMP12 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP12
  • SpecificityRabbit Anti-MMP12, Propeptide Region specifically binds to MMP12 and does not cross-react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc). By immunoblotting, the antibody reacts with a band at 54 kDa (zymogen) but not any of the active forms. Anti-MMP12 recognizes the propeptide region of latent MMP12, which is shed during enzymatic activation. It does not recognize the activated forms of MMP12; thus it can be used to differentiate between the zymogen and the activated enzyme. Higher antibody concentrations may be necessary for non-human samples.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) propeptide.

  • General notesThe antibody is supplied with 50% glycerol to prevent freezing. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000. Detects a band of approximately 54 kDa.

Target

  • FunctionMay be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • Tissue specificityFound in alveolar macrophages but not in peripheral blood monocytes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P39900 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • FormThe presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
    • Alternative names
        EC 3.4.24.65 antibodyHME antibodyMacrophage elastase antibody
        Macrophage metalloelastase antibodyMacrophage Metaloelastase antibodyMatrix metallopeptidase 12 (macrophage elastase) antibodyMatrix Metalloprotease 12 antibodyMatrix metalloproteinase-12 antibodyME antibodyMGC138506 antibodyMME antibodyMMP 12 antibodyMMP-12 antibodyMMP12 antibodyMMP12_HUMAN antibody
      see all

    References for Anti-MMP12 antibody (ab11612)

    ab11612 has not yet been referenced specifically in any publications.

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