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Overview

  • Product nameAnti-MMP12 antibodySee all MMP12 primary antibodies ...
  • Description
    Goat polyclonal to MMP12
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide: C-RPKTSVNLISS, corresponding to internal sequence amino acids 313-323 of Human MMP12 (NP_002417.2)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHuman breast cancer cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab99473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).

Target

  • FunctionMay be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • Tissue specificityFound in alveolar macrophages but not in peripheral blood monocytes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P39900 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • FormThe presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
    • Alternative names
        EC 3.4.24.65 antibodyHME antibodyMacrophage elastase antibody
        Macrophage metalloelastase antibodyMacrophage metaloelastase antibodyMatrix metallopeptidase 12 (macrophage elastase) antibodyMatrix metalloprotease 12 antibodyMatrix metalloproteinase-12 antibodyME antibodyMGC138506 antibodyMME antibodyMMP 12 antibodyMMP-12 antibodyMmp12 antibodyMMP12_HUMAN antibody
      see all

    Anti-MMP12 antibody images

    • Western blot - MMP12 antibody (ab99473)
      Western blot - MMP12 antibody (ab99473)
      Anti-MMP12 antibody (ab99473) at 0.5 µg/ml + Human breast cancer lysate at 35 µg
      developed using the ECL technique

      Predicted band size : 54 kDa

    References for Anti-MMP12 antibody (ab99473)

    ab99473 has not yet been referenced specifically in any publications.

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