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- Secondary antibodies
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Product name
Anti-MMP12 antibody
See all MMP12 products (14) ...
Description
Goat polyclonal to MMP12
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Cow, Dog
Immunogen
Synthetic peptide: C-RPKTSVNLISS, corresponding to internal sequence amino acids 313-323 of Human MMP12 (NP_002417.2)
C-RPKTSVNLISS
Positive control
Human breast cancer cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Ammonium sulphate precipitation followed by antigen affinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP12 antibody (ab99473)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab99473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
Target
Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Tissue specificity
Found in alveolar macrophages but not in peripheral blood monocytes.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP39900
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.4.24.65 antibody
- HME antibody
- Macrophage elastase antibody
see all
Database links
- Entrez Gene: 4321 Human
- Omim: 601046 Human
- SwissProt: P39900 Human
- Unigene: 1695 Human
Anti-MMP12 antibody images:
Western blot - MMP12 antibody (ab99473)
Anti-MMP12 antibody (ab99473) at 0.5 µg/ml + Human breast cancer lysate at 35 µg
developed using the ECL technique
Predicted band size : 54 kDa
References for Anti-MMP12 antibody (ab99473)
ab99473 has not yet been referenced specifically in any publications.
Publishing research using ab99473? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"