MMP12 protein (Human) (ab39311)

Product Name 

MMP12 protein (Human)

Product type 

Proteins and Peptides

Peptide 

Full lenght Human MMP12 protein purified from stimulated human fibroblasts. The enzyme is supplied as a mixture of the zymogen and processed enzyme.

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all MMP12 antibodies

Tested applications 
(see key)

SDS-PAGE, WB

Application notes 
(see key)

Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa. Dilution optimised using Chromogenic detection. MMP12 is quite labile and quickly breaks down to a 21 kDa processed form. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis, metastasis, and atherosclerosis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.

MMP12 was first described in murine macrophages, later in human macrophages, and more recently in other cell types. Also known as metalloelastase, MMP12 is able to degrade elastin, entactin, laminin 1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP12 is often confused with the Serine proteinase, Leukocyte elastase (EC 3.4.21.37) because of similar nomenclature. MMP12 is structurally similar to the classical MMPs (MMP1, MMP3); it contains a propeptide with autoinhibitory cysteine switch site, a well-conserved zinc site, hinge region and hemopexin domain. MMP12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kD, and is quickly activated to the 45 kD form; and this breaks down to cascade of active forms, ending with the common 22 kD form. Stimulated macrophages produce MMP12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts.

This antibody, when stripped of TIMPs, is unstable, and must be stored carefully.

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 10mM MES, 50% Glycerol, 0.025% Brij 35, 5mM Calcium chloride, 100mM Sodium chloride, pH 6.5

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The enzyme is purified from stimulated human fibroblasts, and is free of contaminating MMPs or TIMPs.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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