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Anti-MMP13 antibody [181-15A12] (ab77949)

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Overview

  • Product nameAnti-MMP13 antibody [181-15A12]See all MMP13 primary antibodies ...
  • Description
    Mouse monoclonal [181-15A12] to MMP13
  • SpecificityReacts with precursor and active forms of human MMP13 and does not cross react with human MMP1, 2, 3, 7, 8, 9.
  • Tested applicationsSandwich ELISA, ELISA, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human matrix metalloproteinase 13 (human MMP13)

Properties

Applications

Our Abpromise guarantee covers the use of ab77949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA: Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MMP13 (ab39012). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab39012 as Detection.
ELISA ELISA: Use at an assay dependent dilution.
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P IHC-P: Use at an assay dependent concentration.

Target

  • FunctionDegrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • Tissue specificitySeems to be specific to breast carcinomas.
  • Involvement in diseaseDefects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P45452 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CLG 3 antibodyCLG3 antibodyCollagenase 3 antibody
        Collagenase3 antibodyMANDP1 antibodyMatrix metallopeptidase 13 (collagenase 3) antibodyMatrix Metalloproteinase 13 antibodyMatrix metalloproteinase-13 antibodyMMP 13 antibodyMMP-13 antibodyMmp13 antibodyMMP13_HUMAN antibody
      see all

    Anti-MMP13 antibody [181-15A12] images

    Protocols

    References for Anti-MMP13 antibody [181-15A12] (ab77949)

    ab77949 has not yet been referenced specifically in any publications.

    Publishing research using ab77949 ? Please let us know so that we can cite the reference in this datasheet

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    I was hoping you could provide suggestions/protocols to stain the Netrin 1 (cat#ab39370) and MMP13 (cat#ab77949) antibodies that we've purchased. They will be stained for immunohistochemistry on FFPE human tissues as single stains.

    Thank you for contacting Abcam.

    I have attached the Abcam protocols books that contains some good IHC-P protocols, which contains some good general starting protocols. Specifically for ab39370, we recommend a starting primary antibody concentrati...

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