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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Read our guarantee »Anti-MMP13 antibody [181-15A12]
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Mouse monoclonal [181-15A12] to MMP13
Reacts with precursor and active forms of human MMP13 and does not cross react with human MMP1, 2, 3, 7, 8, 9.
Sandwich ELISA, ELISA, WB, IHC-Pmore details
Reacts with
Human
Recombinant human matrix metalloproteinase 13 (human MMP13)
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: None
Constituents: 4% Protease Free BSA, 0.1M PBS, pH 7.0
Concentration information loading...
Protein G purified
Monoclonal
181-15A12
IgG1
kappa
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Sandwich ELISA - MMP13 antibody [181-15A12] (ab77949)
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Our Abpromise guarantee covers the use of ab77949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MMP13 - Hinge region (ab39012). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab39012 as Detection.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P: Use a concentration of 2.5 µg/ml.
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
Seems to be specific to breast carcinomas.
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP45452
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Sandwich ELISA - MMP13 antibody [181-15A12] (ab77949)
![Sandwich ELISA - MMP13 antibody [181-15A12] (ab77949)](/ps/datasheet/images/77/ab77949/MMP13-Primary-antibodies-ab77949-4.jpg)
Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.
ab77949 has not yet been referenced specifically in any publications.
Publishing research using ab77949? Please let us know so that we can cite the reference in this datasheet
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![Sandwich ELISA - MMP13 antibody [181-15A12] (ab77949)](/ps/datasheet/images/77/ab77949/MMP13-Primary-antibodies-ab77949-4.jpg)
Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.
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