Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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ab39012 has been referenced in 4 publications.
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Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.
All lanes : Anti-MMP13 antibody - Hinge region (ab39012) at 1/3000 dilution
Lane 1 : cell media from human chondrosarcoma (untreated).
Lane 2 : cell media from human chondrosarcoma (IL1 beta treated).
Predicted band size : 54 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
The loading amount is 15 ul/lane, and the condrosarcoma media was concentrated 40x.
ab39012 staining MMP13 in Mouse endochondral precursor (rib cartilage) tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 10% serum for 1 hour at 19ºC. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4ºC. An Alexa Fluor® 647-conjugated Donkey anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.
This image is courtesy of an anonymous Abreview.
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