Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab39012 for help.
There are no answered questions for ab39012
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.
All lanes : Anti-MMP13 antibody - Hinge region (ab39012) at 1/3000 dilution
Lane 1 : cell media from human chondrosarcoma (untreated).
Lane 2 : cell media from human chondrosarcoma (IL1 beta treated).
Predicted band size : 54 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
The loading amount is 15 ul/lane, and the condrosarcoma media was concentrated 40x.
ab39012 staining MMP13 in Mouse endochondral precursor (rib cartilage) tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 10% serum for 1 hour at 19ºC. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4ºC. An Alexa Fluor® 647-conjugated Donkey anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200. DAPI was used for the blue counterstain.
This image is courtesy of an anonymous Abreview.
3
Call 01223 696 000 or contact us