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Western blot - MMP13 antibody (ab39012)
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Product Name
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MMP13 antibody - Hinge region
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See all MMP13 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MMP13 - Hinge region
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Immunogen
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Synthetic peptide based on the hinge region of human MMP13.
(Peptide available as ab44853.)
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Reacts with
(species key)
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Hu, Dog
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Specificity
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ab39012 recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members.
We have a range of domain specific antibodies for this target. For a full list please see all MMP13 antibodies
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Epitope
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ab39012 recognizes the Hinge region of MMP13.
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Predicted molecular weight: 54 kDa. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting. IHC-P: Use at an assay dependent dilution; see Journal of Biological Chemistry 279(26):27633-45. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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cell media from human chondrosarcoma (IL1 beta treated).
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Cellular localization
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Secreted
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Research areas
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Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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Collagenase 3 (MMP13) is a secreted 452 residue protein which is released from cells as aninactive zymogen and activated extracellularly by removal of its propeptide. MMP13 was first identified in tumor cells but has since been found in synoviocytes and normal fibroblasts stimulated by IL6 or a combination of TNF alpha and IL1. Under normal conditions, MMP13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis. In rodents, MMP13 is thought to fill the role of MMP1, which they appear to lack.
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MMP13 antibody - Hinge region (ab39012) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody has been peptide-affinity purified.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP13 antibody - Hinge region is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP13 antibody - Hinge region images, references, products related to ab39012 and other tools.
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MMP13 antibody - Hinge region images:
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 Western blot - MMP13 antibody (ab39012)
All lanes : MMP13 antibody - Hinge region (ab39012) at 1/3000 dilution
Lane 1 : cell media from human chondrosarcoma (untreated).
Lane 2 : cell media from human chondrosarcoma (IL1 beta treated).
Predicted band size : 54 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
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References for MMP13 antibody - Hinge region (ab39012)
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This product has been used in: (two most recent references)
Doehn U et al. RSK is a principal effector of the RAS-ERK pathway for eliciting a coordinate promotile/invasive gene program and phenotype in epithelial cells. Mol Cell 35:511-22 (2009). WB; Dog. PubMed: 19716794
Zijlstra A et al. Collagenolysis-dependent angiogenesis mediated by matrix metalloproteinase-13 (collagenase-3). J Biol Chem 279:27633-45 (2004). PubMed: 15066996
If you publish research using ab39012 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MMP13
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MMP13 antibody - Hinge region - more information
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Customer reviews (feedback) regarding MMP13 antibody - Hinge region |
Customer FAQs regarding MMP13 antibody - Hinge region |
Protocols for MMP13 antibody - Hinge region |
Price and availability of products related to MMP13 antibody - Hinge region |
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MMP13 antibody - Hinge region - related products:
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MMP13 antibody - Hinge region - other tools:
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Contact Abcam with a Technical Enquiry about MMP13 antibody - Hinge region |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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