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All lanes : Anti-MMP13 antibody - Propeptide domain (ab39023) at 1/3000 dilution
Lane 1 : Cell media from human chondrosarcoma (not treated)
Lane 2 : Cell media from human chondrosarcoma (treated with IL1 beta)
Lane 3 : Cell media from human chondrosarcoma (treated with TNF alpha)
Predicted band size : 54 kDa
Observed band size : 56 kDa (why is the actual band size different from the predicted?)
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