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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Anti-MMP13 antibody [VIIIA2 ] (ab3208)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab3208? Please let us know so that we can cite the reference in this datasheet
ab3208 has been referenced in 2 publications.
- Vikman P et al. Lipid-soluble cigarette smoking particles induce expression of inflammatory and extracellular-matrix-related genes in rat cerebral arteries. Vasc Health Risk Manag 5:333-41 (2009). IHC-Fr; Rat. PubMed: 19436652
- Vikman P et al. Transcriptional regulation of inflammatory and extracellular matrix-regulating genes in cerebral arteries following experimental subarachnoid hemorrhage in rats. Laboratory investigation. J Neurosurg 107:1015-22 (2007). IF, IHC; Rat. PubMed: 17977275
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunocytochemistry/ Immunofluorescence - MMP13 antibody [VIIIA2 ] (ab3208)](/ps/datasheet/images/3/ab3208/MMP13-Primary-antibodies-ab3208-1.jpg)
Immunocytochemistry/ Immunofluorescence - MMP13 antibody [VIIIA2 ] (ab3208)
ICC/IF image of ab3208 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3208, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [VIIIA2 ] (ab3208)](/ps/datasheet/images/3/ab3208/MMP13-Primary-antibodies-ab3208-4.jpeg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [VIIIA2 ] (ab3208)
IHC image of ab3208 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3208, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![MMP13 antibody [VIIIA2 ] for Immunohistochemistry (Frozen sections) in Mouse (3208)](/ps/datasheet/images/3/ab3208/MMP13-Primary-antibodies-ab3208-3.jpg)
MMP13 antibody [VIIIA2 ] for Immunohistochemistry (Frozen sections) in Mouse (3208)
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