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Anti-MMP13 antibody [VIIIA2 ] (ab3208)

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Overview

Product name

Anti-MMP13 antibody [VIIIA2 ]
See all MMP13 products (18) ...

Description

Mouse monoclonal [VIIIA2 ] to MMP13

Specificity

ab3208 recognizes pro and acive forms of MMP 13.

Tested applications

IHC-P, IP, IHC-Fr, ICC/IFmore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse

Immunogen

Human recombinant collagenase 3 protein.

Positive control

Conditioned serum free medium from (TPA treated) human chondrosarcoma SW1353 cells. Placenta. Bladder, breast, and ovarian carcinomas.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

VIIIA2

Isotype

IgG1

Light chain type

kappa

Research areas

Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

  • Immunocytochemistry/ Immunofluorescence - MMP13 antibody [VIIIA2 ] (ab3208)Immunocytochemistry/ Immunofluorescence - MMP13 antibody [VIIIA2 ] (ab3208) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [VIIIA2 ] (ab3208)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [VIIIA2 ] (ab3208) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or WB.

Target

Function

Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.

Tissue specificity

Seems to be specific to breast carcinomas.

Involvement in disease

Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.

Sequence similarities

Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionP45452 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CLG 3 antibody
  • CLG3 antibody
  • Collagenase 3 antibody
  • Collagenase3 antibody
  • Matrix Metalloproteinase 13 antibody
  • Matrix metalloproteinase-13 antibody
  • MMP 13 antibody
  • MMP-13 antibody
  • Mmp13 antibody
  • MMP13_HUMAN antibody
see all

Database links

see all

Anti-MMP13 antibody [VIIIA2 ] images:

  Immunocytochemistry/ Immunofluorescence - MMP13 antibody [VIIIA2 ] (ab3208)

Immunocytochemistry/ Immunofluorescence - MMP13 antibody [VIIIA2 ] (ab3208)

ICC/IF image of ab3208 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3208, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [VIIIA2 ] (ab3208)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP13 antibody [VIIIA2 ] (ab3208)

IHC image of ab3208 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3208, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-MMP13 antibody [VIIIA2 ] (ab3208)

This product has been referenced in:

  • Vikman Pet al. Lipid-soluble cigarette smoking particles induce expression of inflammatory and extracellular-matrix-related genes in rat cerebral arteries. Vasc Health Risk Manag 5:333-41 (2009). IHC-Fr; Rat.Read more (PubMed: 19436652) »
  • Vikman Pet al. Transcriptional regulation of inflammatory and extracellular matrix-regulating genes in cerebral arteries following experimental subarachnoid hemorrhage in rats. Laboratory investigation. J Neurosurg 107:1015-22 (2007). IF, IHC; Rat.Read more (PubMed: 17977275) »

See all 2 publications for this product

Publishing research using ab3208? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"