MMP13 antibody (ab9128)

Product Name 

MMP13 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP13

Immunogen 

This polyclonal antibody was raised against recombinant human procollagenase-3 expressed in insect cells

Reacts with 
(species key)

Hu

Specificity 

Based on Western blotting, the antibody reacts with human procollagenase-3.
It does not react to a significant extent with human gelatinase A, gelatinase B, MT1-MMP, and the catalytic domains of MT2-MMP, MT3-MMP, MT4-MMP and MT5-MMP.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/2500 (PMID 18562219).
WB: 1/2500 - 1/8000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

Collagenase 3 (MMP13) is a secreted 452 residue protein which is released from cells as aninactive zymogen and activated extracellularly by removal of its propeptide. MMP13 was first identified in tumor cells but has since been found in synoviocytes and normal fibroblasts stimulated by IL6 or a combination of TNF alpha and IL1. Under normal conditions, MMP13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis. In rodents, MMP13 is thought to fill the role of MMP1, which they appear to lack.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

PBS (20 mM Na-phosphate, pH 7.5, 150 mM NaCl, 0.01 % thimerosal)

Material safety datasheet (MSDS) for this product:
Thimerosal MSDS

Form 

Liquid

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

MMP stands for "Matrix Metalloproteinase"

References for MMP13 antibody (ab9128)

  This product has been used in:

Jones SW et al. Mitogen-activated protein kinase-activated protein kinase 2 (MK2) modulates key biological pathways associated with OA disease pathology. Osteoarthritis Cartilage 17:124-31 (2009). ELISA; Human. PubMed: 18562219

If you publish research using ab9128 please let us know so that we can cite the reference on this datasheet.

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