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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP14 antibody [113-5B7]
See all MMP14 products (19) ...
Description
Mouse monoclonal [113-5B7] to MMP14
Specificity
This antibody cross-reacts slightly with human MMP3.
Tested applications
WB, IP, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Human
Predicted to work with
Sheep, Goat, Cow, Pig, Chinese Hamster, Orangutan
Immunogen
A synthetic peptide (CDGNFDTVAMLRGEM) corresponding to amino acids 319-333 in the hemopexin-like domain of human MMP14
CDGNFDTVAM LRGEM
Positive control
HT1080 or ConA-treated MDMB231 cells
General notes
To induce MMP14 in MDMB231 cells, treat with 1-5 µg/ml ConA for 24-48 h
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.1% BSA, 100mM Sodium phosphate, pH 7
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
113-5B7
Isotype
IgG3
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 10 µg/mlPredicted molecular weight: 66 kDa.
IP: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Target
Function
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
Tissue specificity
Expressed in stromal cells of colon, breast, and head and neck.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP50281
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix metallopeptidase 14 (membrane inserted) antibody
- Matrix metalloproteinase 14 antibody
- Matrix metalloproteinase-14 antibody
see all
Database links
- Entrez Gene: 4323 Human
- Entrez Gene: 17387 Mouse
- Entrez Gene: 81707 Rat
- Omim: 600754 Human
- SwissProt: P50281 Human
- SwissProt: P53690 Mouse
- SwissProt: Q10739 Rat
- Unigene: 2399 Human
see all
References for Anti-MMP14 antibody [113-5B7] (ab58815)
ab58815 has not yet been referenced specifically in any publications.
Publishing research using ab58815? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"