Overview

  • Product nameAnti-MMP14 antibody - Cytoplasmic domainSee all MMP14 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP14 - Cytoplasmic domain
  • SpecificityThis antibody is specific for MMP14, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.).
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the Human MMP14.

  • EpitopeThis antibody binds to the cytoplasmic domain.
  • Positive control
    • DU145 and HDFn cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab28209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa). This antibody detects the pro form at 65kDa and the active form at 64kDa and also detects activation/breakdown products. The extracellular cleaved form is not detected. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
    • Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      The precursor is cleaved by a furin endopeptidase.
    • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P50281 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Matrix metallopeptidase 14 (membrane inserted) antibody
      • Matrix metalloproteinase 14 antibody
      • Matrix metalloproteinase-14 antibody
      • Membrane type 1 Matrix Metalloproteinase antibody
      • Membrane type 1 metalloprotease antibody
      • Membrane type matrix metalloproteinase 1 antibody
      • Membrane-type matrix metalloproteinase 1 antibody
      • Membrane-type-1 matrix metalloproteinase antibody
      • MMP 14 antibody
      • MMP X1 antibody
      • MMP-14 antibody
      • MMP-X1 antibody
      • Mmp14 antibody
      • MMP14_HUMAN antibody
      • MMPX1 antibody
      • MT MMP 1 antibody
      • MT-MMP 1 antibody
      • MT1 MMP antibody
      • MT1-MMP antibody
      • MT1MMP antibody
      • MTMMP 1 antibody
      • MTMMP1 antibody
      • MTMMP1 antibody
      see all

    References for Anti-MMP14 antibody - Cytoplasmic domain (ab28209)

    This product has been referenced in:
    • Lafleur MA  et al. Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell 20:2030-40 (2009). WB . Read more (PubMed: 19211836) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (MCF-7)
    Loading amount 20 µg
    Specification MCF-7
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

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    Verified customer

    Submitted Dec 17 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"