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Overview

  • Product nameAnti-MMP14 antibody - Cytoplasmic domainSee all MMP14 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP14 - Cytoplasmic domain
  • SpecificityThis antibody is specific for MMP14, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.).
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the Human MMP14.

  • EpitopeThis antibody binds to the cytoplasmic domain.
  • Positive controlDU145 and HDFn cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab28209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa). This antibody detects the pro form at 65kDa and the active form at 64kDa and also detects activation/breakdown products. The extracellular cleaved form is not detected. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
    • Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications      The precursor is cleaved by a furin endopeptidase.
    • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

      • Target information above from: UniProt accession P50281 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          Matrix metallopeptidase 14 (membrane inserted) antibodyMatrix metalloproteinase 14 antibodyMatrix metalloproteinase-14 antibody
          Membrane type 1 Matrix Metalloproteinase antibodyMembrane type 1 metalloprotease antibodyMembrane type matrix metalloproteinase 1 antibodyMembrane-type matrix metalloproteinase 1 antibodyMembrane-type-1 matrix metalloproteinase antibodyMMP 14 antibodyMMP X1 antibodyMMP-14 antibodyMMP-X1 antibodyMmp14 antibodyMMP14_HUMAN antibodyMMPX1 antibodyMT MMP 1 antibodyMT-MMP 1 antibodyMT1 MMP antibodyMT1-MMP antibodyMT1MMP antibodyMTMMP 1 antibodyMTMMP1 antibodyMTMMP1 antibody
        see all

      References for Anti-MMP14 antibody - Cytoplasmic domain (ab28209)

      This product has been referenced in:
      • Lafleur MA  et al. Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell 20:2030-40 (2009). WB . Read more (PubMed: 19211836) »

      See 1 Publication for this product

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      Western blot abreview for Anti-MMP14 antibody - Cytoplasmic domain

       Average
      Abreviews
      Application Western blot
      Sample Human Cell lysate - whole cell (MCF-7)
      Loading amount 20 µg
      Specification MCF-7
      Gel Running Conditions Reduced Denaturing (10%)
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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      Submitted Dec 17 2008

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"