Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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This customer raises an enquiry about ab51074 (Anti-MMP14 antibody [EP1264Y]), he wants to know the more information about this immunogen, could you please offer any information to him (ex. N-terminal or C-terminal), thanks for your kindly help. |
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Thank you for contacting us. |
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Question 1 : Anti-MMP14 antibody [EP1264Y] (ab51074) |
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rabbit monoclonal light chain: kappa or lambda? |
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Thank you for contacting us. I have heard back from the lab: At current time, for ab51074, we have not determined the exact light chain type for this antibody. However, based on the published literature regarding rabbit monoclonals, it is most likely a kappa light chain: www.ncbi.nlm.nih.gov/pmc/articles/PMC3102571 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution + Recombinant protein (1ng)
Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size : 66 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Ab51074 (1:100) staining human MMP14 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
ab51074 at 1/500 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.
This image is courtesy of an anonymous Abreview
Overlay histogram showing HT1080 cells stained with ab51074 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51074, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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