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RabMAbs (2010-1)

Anti-MMP14 antibody [EP1264Y] (ab51074)

Abreviews (9)Q&A (7)Specific References (11)

Overview

  • Product nameAnti-MMP14 antibody [EP1264Y]See all MMP14 primary antibodies ...
  • Description
    Rabbit monoclonal [EP1264Y] to MMP14
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt, IP, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in human MMP14.

  • Positive controlIHC: Human breast carcinoma tissue. WB: recombinant protein
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab51074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/100 - 1/250.
ICC/IF ICC/IF: 1/100 - 1/250.
Flow Cyt Flow Cyt: 1/50 - 1/100. (paraformaldehyde or methanol fixed cells)
IP IP: 1/100.
WB WB: 1/2000. Predicted molecular weight: 66 kDa.

Target

  • FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

    • Target information above from: UniProt accession P50281 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      Matrix metallopeptidase 14 (membrane inserted) antibodyMatrix metalloproteinase 14 antibodyMatrix metalloproteinase-14 antibody
      Membrane type 1 matrix metalloproteinase antibodyMembrane type 1 metalloprotease antibodyMembrane type matrix metalloproteinase 1 antibodyMembrane-type matrix metalloproteinase 1 antibodyMembrane-type-1 matrix metalloproteinase antibodyMMP 14 antibodyMMP X1 antibodyMMP-14 antibodyMMP-X1 antibodyMmp14 antibodyMMP14_HUMAN antibodyMMPX1 antibodyMT MMP 1 antibodyMT-MMP 1 antibodyMT1 MMP antibodyMT1-MMP antibodyMT1MMP antibodyMTMMP 1 antibodyMTMMP1 antibody
    see all

Anti-MMP14 antibody [EP1264Y] images

  • Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Western blot - Anti-MMP14 antibody [EP1264Y] (ab51074)
    Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution + Human spleen lysate

    Secondary
    Goat anti-Rabbit HRP labeled. at 1/2000 dilution

    Predicted band size : 66 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Paraffin-embedded sections) - MMP14 antibody [EP1264Y] (ab51074)
    Immunohistochemistry (Paraffin-embedded sections) - MMP14 antibody [EP1264Y] (ab51074)
    Ab51074 (1:100) staining human MMP14 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP14 antibody [EP1264Y] (ab51074)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP14 antibody [EP1264Y] (ab51074)This image is courtesy of an anonymous Abreview
    ab51074 at 1/500 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.

    See Abreview

  • Flow Cytometry - MMP14 antibody [EP1264Y] (ab51074)
    Flow Cytometry - MMP14 antibody [EP1264Y] (ab51074)
    Overlay histogram showing HT1080 cells stained with ab51074 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51074, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Protocols

References for Anti-MMP14 antibody [EP1264Y] (ab51074)

This product has been referenced in:
  • Urso ML  et al. Adenosine A3 receptor stimulation reduces muscle injury following physical trauma and is associated with alterations in the MMP/TIMP response. J Appl Physiol 112:658-70 (2012). WB ; Mouse . Read more (PubMed: 22114177) »
  • Bialek J  et al. Relaxin enhances the collagenolytic activity and in vitro invasiveness by upregulating matrix metalloproteinases in human thyroid carcinoma cells. Mol Cancer Res 9:673-87 (2011). ICC/IF ; Human . Read more (PubMed: 21493587) »

See all 11 Publications for this product

Publishing research using ab51074 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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Displaying 1 - 10 of 16 results for Abreviews and Q&A

10
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Western blot abreview for Anti-MMP14 antibody [EP1264Y]

 Good
Abreviews
Application Western blot
Sample Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount 40 µg
Specification Heart, Left Ventricle
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Dec 28 2012

Phone call requesting the concentration of the following antibodies and lots: ab52897, GR5286-6; ab39012, 275628; ab51074, GR36169-4; ab39206, GR11688-1; ab15323, GR59236-1.

Thank you for contacting us and your interest in our products. As discussed over the phone, I have checked the concentration of the antibodies and lot numbers you provided. The concentrations are as follows:

ab52897, GR5286-6, 0.165 mg/ml;
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Inquiry: Hi. We have had difficulties in finding a good rabbit antibody for MMP14. I am interested in ordering your rabbit monoclonal MMP14 antibody, however I would need to know at which region of the protein the epitope is sited. Is it at the catalytic ...

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Thank you for your enquiry.

I can confirm that the immunogen sequence for MMP14 antibody [EP1264Y] (ab51074) is derived from somewhere within amino acids 150-180 (extracellular region) of human MMP-14 which is in the extracellular domain. I am ...

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I realized I forgot to mention the lot#. This is GR83749-1

Could you also tell me the concentration of antibody MMP-14, catalog# ab51074 with lot# GR87560-1.

Thanks again,

Thank you for your enquiry.

I can confirm that ab51074 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the...

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I am interested in using your anti-MMP14 antibody [EP1264Y](ab51074).
Would it be possible to find out what residues or region of MMP14 this antibody was immunized against.

Thank you for contacting Abcam.

The immunogen sequence that was used to generate ab51074 was:
*

If there is anything else I can help you with, please let me know.

Western blot abreview for Anti-MMP14 antibody [EP1264Y]

 Good
Abreviews
Application Western blot
Sample Human Cell lysate - other (Foreskin Fibroblasts)
Loading amount 1e+007 cells
Specification Foreskin Fibroblasts
Gel Running Conditions Reduced Denaturing (7.5% acrylamide)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Username

Verified customer

Submitted Jun 09 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-MMP14 antibody [EP1264Y]

 Excellent
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Foreskin fibroblasts)
Specification Foreskin fibroblasts
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Username

Verified customer

Submitted Jun 08 2012

This customer raises an enquiry about ab51074 (Anti-MMP14 antibody [EP1264Y]), he wants to know the more information about this immunogen, could you please offer any information to him (ex. N-terminal or C-terminal), thanks for your kindly help.

Thank you for contacting us.


According to our internal records, this immunogen isnot from the N-terminus. In addition, itwill recognise pro and mature forms.


I hope this information is helpful to you. Please do not hesitat...

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Question 1 : Anti-MMP14 antibody [EP1264Y] (ab51074)
How much is the concentration and how many the total volume of them?


ab51074 : The unit size is 100µl, see http://www.abcam.com/ab51074.This is a Tissue culture supernatant so the concentration is not determined. See http://www.abcam.com/index.html?pageconfig=technicalfaqs#8

rabbit monoclonal light chain: kappa or lambda?

Thank you for contacting us. I have heard back from the lab: At current time, for ab51074, we have not determined the exact light chain type for this antibody. However, based on the published literature regarding rabbit monoclonals, it is most likely ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"