Anti-MMP14 antibody [EP1264Y] (ab51074)

  Western blot - MMP14 antibody [EP1264Y] (ab51074)

Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution + Recombinant protein (1ng)

Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution

Predicted band size : 66 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - MMP14 antibody [EP1264Y] (ab51074)

Ab51074 (1:100) staining human MMP14 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP14 antibody [EP1264Y] (ab51074)

ab51074 at 1/500 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.

This image is courtesy of an anonymous Abreview

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  Flow Cytometry - MMP14 antibody [EP1264Y] (ab51074)

Overlay histogram showing HT1080 cells stained with ab51074 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51074, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.