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All lanes : Anti-MMP14 antibody - Propeptide domain (ab38969)
Lane 1 : Cell lysates from human dermal fibroblasts neonatal (no treatement)
Lane 2 : Cell lysates from human dermal fibroblasts neonatal (treated with II1beta)
Performed under reducing conditions.
Predicted band size : 66 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
ab38969 staining Propeptide domain in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton x100 and no blocking was done. Samples were incubated with primary antibody diluted to 10µg/ml for 1 hour at 20°C. An Alexa Fluor®555-conjugated donkey polyclonal to goat IgG was used undiluted as secondary antibody.
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