Overview

  • Product nameAnti-MMP14 antibodySee all MMP14 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP14
  • Specificityab53712 detects endogenous levels of total MMP14 protein
  • Tested applicationsICC/IF, WB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MMP14 aa 471-520 (C terminal).
    Database link: P50281

  • Positive control
    • Human breast carcinoma tissue. K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab53712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23326364
WB Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.

Target

  • FunctionSeems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • Tissue specificityExpressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P50281 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all

Anti-MMP14 antibody images

  • All lanes : Anti-MMP14 antibody (ab53712) at 1 µg/ml

    Lane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 66 kDa
    Observed band size : 66 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • ab53712, at a 1/50 dilution, minus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.ab53712, at a 1/50 dilution, minus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.
  • ab53712, at a 1/50 dilution, plus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemstry, Paraffin embedded tissue.ab53712, at a 1/50 dilution, plus immunising peptide, staining Human MMP14 in Breast Carcinoma, using Immunohistochemstry, Paraffin embedded tissue.

References for Anti-MMP14 antibody (ab53712)

This product has been referenced in:
  • Janssens E  et al. Matrix metalloproteinase 14 in the zebrafish: an eye on retinal and retinotectal development. PLoS One 8:e52915 (2013). WB, IHC-P ; Zebrafish . Read more (PubMed: 23326364) »
  • Zamilpa R  et al. CC chemokine receptor 5 deletion impairs macrophage activation and induces adverse remodeling following myocardial infarction. Am J Physiol Heart Circ Physiol 300:H1418-26 (2011). WB ; Mouse . Read more (PubMed: 21297029) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
Loading amount 100000 cells
Specification rat vascular smooth muscle cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2013

Thank you for your inquiry.

Please find the SDS in the attachment. The SDS can now be downloaded also from the datasheet directly. Please follow this link: http://www.abcam.com/index.html?datasheet=53712 (or use the following: http://www.abca...

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Thank you for your inquiry.

The antibody you mentioned is a polyclonal antibody. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope. Therefore for polyclonal antibodies the epitopes a...

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Thank you for contacting us.

I am sorry to hear that you are encountering problems with these antibodies in WB.

I agree thatab38898 is giving you poor results, and this could be a due to a faulty vial or lot.

As for the M...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lymphoma)
Specification lymphoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"