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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP15 antibody
See all MMP15 products (8) ...
Description
Rabbit polyclonal to MMP15
Specificity
This antibody detects endogenous levels of total MMP15 protein.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic MMP15 peptide (Human)
Positive control
Extracts from MDA-MB-435 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP15 antibody (ab53770)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
WB: 1/500 - 1/1000.Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
Target
Function
Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
Tissue specificity
Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Membrane.
Target information above from: UniProt accessionP51511
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MMP 15 antibody
- Matrix metallopeptidase 15 membrane inserted antibody
- Matrix metalloproteinase 15 antibody
see all
Database links
- Entrez Gene: 4324 Human
- Entrez Gene: 17388 Mouse
- Entrez Gene: 291848 Rat
- Omim: 602261 Human
- SwissProt: P51511 Human
- SwissProt: O54732 Mouse
- Unigene: 80343 Human
- Unigene: 217116 Mouse
Anti-MMP15 antibody images:
Western blot - MMP15 antibody (ab53770)
All lanes : Anti-MMP15 antibody (ab53770) at 1/500 dilution
Lane 1 : Extracts from MDA-MB-435 cells
Lane 2 : Extracts from MDA-MB-435 cells, plus peptide
Predicted band size : 76 kDa
Observed band size : 76 kDa
References for Anti-MMP15 antibody (ab53770)
ab53770 has not yet been referenced specifically in any publications.
Publishing research using ab53770? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"