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Western blot - MMP15 antibody (ab53770)
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Product Name
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MMP15 antibody
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See all MMP15 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MMP15
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Immunogen
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Synthetic MMP15 peptide (Human)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody detects endogenous levels of total MMP15 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from MDA-MB-435 cells
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Cellular localization
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Membrane; single pass type I membrane protein.
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Research areas
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Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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The mammalian matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins.
MMP15, first described as MTMMP2, is a metalloproteinase with a type I transmembrane domain. Initially reported in lung tissue, MMP15 message was also seen in testis, intestines, colon, heart, liver, kidney, pancreas and muscle. Western blots showed bands at 72 kD, 48 kD and 44 kD. Subsequent publications have focused on message, and found MMP15 in a wide range of tumor cell lines, as well as the synovial membrane from patients with rheumatoid arthritis. MMP15 is usually found in much lower levels than the more ubiquitous MMP14 (MT1MMP). MMP15 may be shed into the culture media when activated, but it is unclear if the shed form is proteolytically active. Cell lysates are the best source for Western blotting of MMP15.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP15 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP15 antibody images, references, products related to ab53770 and other tools.
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MMP15 antibody images:
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 Western blot - MMP15 antibody (ab53770)
All lanes : MMP15 antibody (ab53770) at 1/500 dilution
Lane 1 : Extracts from MDA-MB-435 cells
Lane 2 : Extracts from MDA-MB-435 cells, plus peptide
Predicted band size : 76 kDa
Observed band size : 76 kDa
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Search PubMed (MEDLINE) for references to MMP15
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MMP15 antibody - more information
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Customer reviews (feedback) regarding MMP15 antibody |
Customer FAQs regarding MMP15 antibody |
Protocols for MMP15 antibody |
Price and availability of products related to MMP15 antibody |
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MMP15 antibody - related products:
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MMP15 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MMP15 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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