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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP16 antibody - Aminoterminal end active enzyme
See all MMP16 products (9) ...
Description
Rabbit polyclonal to MMP16 - Aminoterminal end active enzyme
Specificity
The antibody binds to MT3-MMP, but does not cross react with the other MTMMP or MMP family members (MMP-14, MMP-15, MMP-17, MMP-1, MMP-2, MMP-3, MMP-9, etc).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide of based on the Human sequence of the aminoterminal end of furin-cleaved active MMP-16.
(Peptide available as ab43114.)
Positive control
MT3-MMP has been reported to be elevated in several tumor cell lines, and is constituitively produced by some normal cell lines.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP16 antibody - Aminoterminal end active enzyme (ab39694)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: 1/1000 - 1/5000. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for non-human samples. When used against the reduced protein, ab39694 identifies bands at 65 kDa and 63 kDa (the pro-form and active form), as well as activation/breakdown products. Treatment of cells with Concanavolin-A or the phorbol ester TPA stimulates production of MT3-MMP in some cell types, and the enzyme can be recovered in cell lysates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Tissue specificity
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Developmental stage
Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Cell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
Target information above from: UniProt accessionP51512
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix Metalloproteinase 16 antibody
- Matrix metalloproteinase-16 antibody
- Membrane type 3 matrix metalloproteinase antibody
see all
Database links
- Entrez Gene: 4325 Human
- Omim: 602262 Human
- SwissProt: P51512 Human
- Unigene: 492187 Human
- Unigene: 546267 Human
Anti-MMP16 antibody - Aminoterminal end active enzyme images:
Western blot - MMP16 antibody - Aminoterminal end active enzyme (ab39694)
All lanes : Anti-MMP16 antibody - Aminoterminal end active enzyme (ab39694) at 1/1000 dilution
Lane 1 : SF treated A2058 Lysate
Lane 2 : TPA treated A2058 Lysate
Lane 3 : IL1-alpha treated A2058 Lysate
Lane 4 : TNF-alpha treated A2058 Lysate
Lysates/proteins at 5 µl per lane.
Predicted band size : 63 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-MMP16 antibody - Aminoterminal end active enzyme (ab39694)
ab39694 has not yet been referenced specifically in any publications.
Publishing research using ab39694? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"