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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP16 antibody - Cytoplasmic domain
See all MMP16 products (9) ...
Description
Rabbit polyclonal to MMP16 - Cytoplasmic domain
Specificity
Does not cross react with other MTMMP or MMP family members (MMP-14, MMP-15, MMP-17, MMP1, MMP2, MMP3, MMP-9, etc).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the cytoplasmic domain of human MMP16.
Epitope
This antibody binds to the Cytoplasmic tail of MMP16. For the antibody to gain access to the epitope, cells or tissue need to be permeabilized.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP16 antibody - Cytoplasmic domain (ab38967)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab38967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: A recommended starting concentration for Western blots is 1:2,000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. When used against the reduced protein, Ab38967 identifies bands at 65 Kd and 63 Kd (the pro-form and active form), as well as activation/breakdown products. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Tissue specificity
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Developmental stage
Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Cell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
Target information above from: UniProt accessionP51512
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix Metalloproteinase 16 antibody
- Matrix metalloproteinase-16 antibody
- Membrane type 3 matrix metalloproteinase antibody
see all
Database links
- Entrez Gene: 4325 Human
- Omim: 602262 Human
- SwissProt: P51512 Human
- Unigene: 492187 Human
- Unigene: 546267 Human
Anti-MMP16 antibody - Cytoplasmic domain images:
Western blot - MMP16 antibody - Cytoplasmic domain (ab38967)
All lanes : Anti-MMP16 antibody - Cytoplasmic domain (ab38967) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from human prostate carcinoma 22Rv-1 cells (1:8) (treated with SF)
Lane 2 : Whole cell lysate prepared from human prostate carcinoma 22Rv-1 cells (1:8) (treated with TPA)
Lane 3 : Whole cell lysate prepared from human epithelial carcinoma A-431 cells (1:5) (treated with SF)
Lane 4 : Whole cell lysate prepared from human epithelial carcinoma A-431 cells (1:5) (treated with TPA)
Lysates/proteins at 15 µl per lane.
Predicted band size : 70 kDa
References for Anti-MMP16 antibody - Cytoplasmic domain (ab38967)
ab38967 has not yet been referenced specifically in any publications.
Publishing research using ab38967? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"