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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP16 antibody
See all MMP16 products (9) ...
Description
Rabbit polyclonal to MMP16
Specificity
This antibody specifically recognises MMP16, and does not cross-react with MMP1, 2, 3, 7, 8, 9 or 10.
Tested applications
WB, ELISA, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 167-181 of Human MMP 16.
Positive control
PMA stimulated A431 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Proclin
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP16 antibody (ab5709)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2.0 µg/ml.Detects a band of approximately 65 kDa.
ELISA: Use a concentration of 0.1 - 1.0 µg/ml.
IP: Use 3-5µg for 106 cells.
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Tissue specificity
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Developmental stage
Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Cell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
Target information above from: UniProt accessionP51512
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix Metalloproteinase 16 antibody
- Matrix metalloproteinase-16 antibody
- Membrane type 3 matrix metalloproteinase antibody
see all
Database links
- Entrez Gene: 4325 Human
- Entrez Gene: 17389 Mouse
- Entrez Gene: 65205 Rat
- Omim: 602262 Human
- SwissProt: P51512 Human
- SwissProt: Q9WTR0 Mouse
- SwissProt: O35548 Rat
- Unigene: 492187 Human
see all
Anti-MMP16 antibody images:
Western blot - MMP16 antibody (ab5709)
PMA stimulated A431 cell lysate was probed with rabbit anti MMP16 at 1/500, a major band was detected. This band was abolished upon addition of the blocking peptide.
References for Anti-MMP16 antibody (ab5709)
ab5709 has not yet been referenced specifically in any publications.
Publishing research using ab5709? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"