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Western blot - MMP16 antibody (ab5709)

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Product Name
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MMP16 antibody
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See all MMP16 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MMP16
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Immunogen
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Synthetic peptide, corresponding to amino acids 167-181 of Human MMP 16.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat (Due to sequence homology)
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Specificity
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This antibody specifically recognises MMP16, and does not cross-react with MMP1, 2, 3, 7, 8, 9 or 10.
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Tested applications
(see key)
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ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: Use at a concentration of 0.1 - 1.0 µg/ml. IP: Use 3-5µg for 106 cells. WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 65 kDa.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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PMA stimulated A431 whole cell lysate.
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Cellular localization
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Secreted protein; extracellular space; extracellular matrix
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zinc endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. MMP16 induces the activation of pro gelatinase A (MMP2). It was identified as a membrane bound Metalloproteinase in normal and tumor cell lines. MMP16 is similar to the other MtMMPs; it contains a furin cleavage site, is membrane bound, and contains a cytoplasmic tail (MT4MMP lacks the tail, and may not be intercalated into the membrane). MMP16 is also known to be "shed" from the membrane in a soluble form. MT1MMP is known to function in activating a number of MMPs, chiefly MMP2, but that role has not been well described for the other MTMMPs. MMP16 has been reported to be elevated in several tumor cell lines, and is constituitively produced by some normal cell lines.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Proclin Constituents: PBS, pH 7.4
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP16 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP16 antibody images, references, products related to ab5709 and other tools.
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MMP16 antibody images:
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Western blot - MMP16 antibody (ab5709)
PMA stimulated A431 cell lysate was probed with rabbit anti MMP16 at 1/500, a major band was detected. This band was abolished upon addition of the blocking peptide.
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Search PubMed (MEDLINE) for references to MMP16
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MMP16 antibody - more information
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Customer reviews (feedback) regarding MMP16 antibody |
Customer FAQs regarding MMP16 antibody |
Protocols for MMP16 antibody |
Price and availability of products related to MMP16 antibody |
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MMP16 antibody - related products:
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MMP16 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MMP16 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"