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Read our guarantee »Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Anti-MMP16 antibody
See all MMP16 products (9) ...
Rabbit polyclonal to MMP16
This antibody specifically recognises MMP16, and does not cross-react with MMP1, 2, 3, 7, 8, 9 or 10.
WB, ELISA, IPmore details
Reacts with
Human
Predicted to work with
Mouse, Rat
Synthetic peptide, corresponding to amino acids 167-181 of Human MMP 16.
PMA stimulated A431 whole cell lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Proclin
Constituents: PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Western blot - MMP16 antibody (ab5709)
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Our Abpromise guarantee covers the use of ab5709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2.0 µg/ml.Detects a band of approximately 65 kDa.
ELISA: Use a concentration of 0.1 - 1.0 µg/ml.
IP: Use 3-5µg for 106 cells.
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
The precursor is cleaved by a furin endopeptidase.
Cell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
Target information above from: UniProt accessionP51512
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - MMP16 antibody (ab5709)

PMA stimulated A431 cell lysate was probed with rabbit anti MMP16 at 1/500, a major band was detected. This band was abolished upon addition of the blocking peptide.
ab5709 has not yet been referenced specifically in any publications.
Publishing research using ab5709? Please let us know so that we can cite the reference in this datasheet
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